NEBNext^® Ultra™ RNA Library Prep Kit for Illumina^®

Features

• Low input amounts (as low as 10ng total RNA, purified mRNA or ribosomal-depleted RNA)
• Fast workflow (5 – 5.5 hours), with minimal hands-on time
• Robust, reliable performance
• Ultra high fidelity amplification with minimized GC bias

Companion Products

• NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat)

1. Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB #E7490 and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530)
2. Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat), NEB #E6310 and NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530)
3. Protocol for use with Purified mRNA or Ribosome Depleted RNA - NEBNext Ultra RNA Library Prep Kit for Illumina (E7530)

• Getting Started with RNA-Sequencing (RNA-Seq)

1. What is the difference between the NEBNext Ultra Directional RNA library prep kit for Illumina (E7420) and the NEBNext Ultra RNA library prep kit for Illumina (E7530)?
2. What is the starting material I need to use when preparing libraries using the NEBNext Ultra Directional RNA kit?
3. Where do I have to start the protocol if I have purified mRNA or Ribosomal-depleted RNA?
4. Which kit can I use to isolate Poly (A) mRNA from Total RNA?
5. Does the kit provide adaptor and primers?
6. How do I know the concentration of my index primers? 
7. Which NEBNext Oligos can be used with this library prep kit?
8. Can I use this NEBNext kit with adaptors and primers from other vendors than NEB?

• NEBNext® RNA Library Prep Kit Troubleshooting Guide (NEB #E7760, E7765, E7770, E7775, E7420, E7530)

Product Citation Tool

Additional Citations

• Fustin, J.-M. et al. (2013) RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock Cell; 155(4), 793-806. PubMedID: 24209618 , DOI: 10.1016/j.cell.2013.10.026

• Huang, L., Jin, J., Deighan, P., Liner, E., McReynolds, L. and Lieberman, J. (2013) . Efficient and specific gene knockdown by small interfering RNAs produced in bacteria Nat Biotechnol..; 31(4), PubMedID: 23475073 , DOI: 10.1038/nbt.2537

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7530S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7530S_v1_10010221
• E7530L_v1_10013234
• E7530L_v1_10016097
• E7530L_v1_10018657
• E7530S_v1_10018695
• E7530L_v1_10025002
• E7530S_v1_10026780
• E7530L_v1_10030619
• E7530S_v1_10031919
• E7530L_v1_10034747
• E7530S_v1_10036793
• E7530S_v1_10041072
• E7530L_v1_10044552
• E7530S_v1_10019109
• E7530L_v1_10051551
• E7530S_v1_10051042
• E7530S_v1_10051824

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Nuclease-free Water
• NEBNext End Prep Enzyme Mix
• NEBNext End Repair Reaction Buffer
• Blunt/TA Ligase Master Mix
• NEBNext First Strand Synthesis Reaction Buffer
• Random Primers
• ProtoScript® II Reverse Transcriptase
• Murine RNase Inhibitor
• NEBNext Second Strand Synthesis Enzyme Mix
• NEBNext Second Strand Synthesis Reaction Buffer
• NEBNext® Q5® Hot Start HiFi PCR Master Mix

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Licenses

This product is covered by one or more patents.

This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.