This original version of the Ultra™ RNA Library Prep Kit enables non-directional RNA library preparation in a streamlined workflow. However, we now recommend use of the more recent NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts.
The NEBNext® Ultra™ RNA Library Prep Kit for Illumina® contains reagents for preparation of non-directional RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion reagents and poly(A) mRNA isolation reagents are not included in the kit and are available separately.
Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
NEBNext Ultra™ RNA provides even sequence coverage across transcripts for a wide range of inputs
10 ng, 100ng or 1 μg of Total RNA (Universal Human Reference RNA, Agilent #740000) was used as input for libraries, following the NEBNext Ultra™ RNA protocol and using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were sequenced (single end reads) on the Illumina platform (GAIIx). Good coverage across expressed transcript was obtained for each input amount. Ultra Non-directional RNA Library Preparation for Illumina
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied
The following reagents are supplied with this product:
Huang, L., Jin, J., Deighan, P., Liner, E., McReynolds, L. and Lieberman, J.
(2013)
. Efficient and specific gene knockdown by small interfering RNAs produced in bacteria Nat Biotechnol..;
31(4), PubMedID: 23475073 , DOI: 10.1038/nbt.2537
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Licenses
This product is covered by one or more patents.
This product is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645 and corresponding patents in other countries for use only in: (a) standard (not real-time) PCR in the research field only, but not real time PCR or digital PCR; (b) real-time PCR for use as a library preparation quantitation tool in Next Generation Sequencing workflows; (c) any in-vitro diagnostics applications, except for applications using real-time PCR or digital PCR; and (d) any non-PCR applications in DNA sequencing, isothermal amplification, and the production of synthetic DNA.
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