Note that RNA Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext rRNA Depletion Kit with Sample Purification Beads (NEB #E6350).
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g. FFPE RNA) and with low input amounts. The NEBNext rRNA Depletion kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.
Suitable for low-quality (e.g. FFPE) or high-quality RNA
Compatible with a broad range of input amounts: 10 ng - 1 μg
Fast workflow: 2 hours, with less than 10 minutes hands-on time
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) employs an RNaseH-based method (1,2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
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The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
This product and its use are subject to one or more issued and/or pending U.S. and foreign patent applications. Commercial use for the determination, prediction and prognosis of cancer, or response to therapy for cancer, in humans or animals may require a license. Please contact [email protected] for more information.
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