An updated version of this kit (NEB #E7400) is available for superior performance.
Note that RNA Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext rRNA Depletion Kit v2 with Sample Purification Beads (E7405), but the NEBNext rRNA Depletion Kit with Sample Purification Beads (NEB #E6350) is also available.
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA. Efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples, but this can be especially challenging with low quality RNA (e.g. FFPE RNA) and with low input amounts. The NEBNext rRNA Depletion kit employs the efficient RNase H method, as well as complete probe tiling of rRNA, thereby ensuring that even degraded rRNA is hybridized and subsequently removed.
Suitable for low-quality (e.g. FFPE) or high-quality RNA
Compatible with a broad range of input amounts: 10 ng - 1 μg
Fast workflow: 2 hours, with less than 10 minutes hands-on time
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) employs an RNaseH-based method (1,2) to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g. FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications.
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