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Home NGS Sample Prep & Target Enrichment Products NEBNext^® Poly(A) mRNA Magnetic Isolation Module

NEBNext^® Poly(A) mRNA Magnetic Isolation Module

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

Catalog #	Concentration	Size
E7490S	Not Applicable	24 reactions
E7490L	Not Applicable	96 reactions
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Product Information

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

E7490S

NEBNext RNA Binding Buffer (2X)	E7492AVIAL	4	1 x 7.2 ml	Not Applicable
NEBNext Wash Buffer	E7493AVIAL	4	1 x 28.8 ml	Not Applicable
Nuclease-free Water	E7495AVIAL	4	1 x 1.2 ml	Not Applicable
NEBNext Tris Buffer	E7496AVIAL	4	1 x 6 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AVIAL	4	1 x 0.48 ml	Not Applicable

E7490L

NEBNext RNA Binding Buffer (2X)	E7492AAVIAL	4	1 x 28.8 ml	Not Applicable
NEBNext Wash Buffer	E7493AAVIAL	4	2 x 57.6 ml	Not Applicable
Nuclease-free Water	E7495AAVIAL	4	1 x 4.8 ml	Not Applicable
NEBNext Tris Buffer	E7496AAVIAL	4	1 x 24 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AAVIAL	4	1 x 1.92 ml	Not Applicable

This product is related to the following categories:: RNA Library Prep Products

This product can be used in the following applications:: RNA Modification,; RNA-seq,; RNA For Illumina,; RNA Purification and Isolation,

Advantages and Features

Application Features
Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.
Properties & Usage
Materials Required but not Supplied
- 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
- Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
- Thermal cycler or heat block
- Bioanalyzer^® (Agilent Technologies, Inc.) or similar instrument and consumables.

Protocols, Manuals & Usage
- Protocols
  Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)
  
  Protocol for Enrichment of mRNAs, excluding Globin mRNA, from Whole Blood Total RNA
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
  
  manualE7490
- Usage Guidelines
  Getting Started with RNA-Sequencing (RNA-Seq)
FAQs & Troubleshooting
- FAQs
  Can I isolate poly(A)⁺ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
  
  Do I need two rounds of binding?
  
  Can I use the Oligo (dT)₂₅ Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
Citations & Technical Literature
- Citations
  
  NEBNext Product Citations Search Tool
  
  Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  E7490S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  E7490G_v0_10010544
  
  E7490L_v0_10012676
  
  E7490S_v0_10011311
  
  E7490G_v0_10013797
  
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  E7490S_v1_10054401
  
  E7490G_v0_10070797
  
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  E7490S_v1_10071608
  
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  E7490L_v1_10061867
  
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  E7490L_v1_10080706
  
  E7490S_v1_10080973
  
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  E7490S_v1_10106917
  
  E7490G_v0_10071724
  
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  E7490L_v1_10110898
  
  E7490S_v1_10110353
  
  E7490L_v1_10112752
  
  E7490G_v0_10092659
  
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  E7490S_v1_10116719
  
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  E7490S_v1_10144645
  
  E7490L_v1_10115769
  
  E7490L_v1_10151189
  
  E7490S_v1_10150134
  
  E7490S_v1_10115759
  
  E7490S_v1_10154850
  
  E7490L_v1_10155185
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext RNA Binding Buffer (2X)
  
  NEBNext Wash Buffer
  
  Nuclease-free Water
  
  NEBNext Tris Buffer
  
  NEBNext Oligo d(T)25 Beads
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please email us at busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Licenses
  This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.

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Resources

NEBNext^® Poly(A) mRNA Magnetic Isolation Module

Reagents Supplied

Application Features

Materials Required but not Supplied

NEBNext Product Citations Search Tool

Specifications & Change Notifications

Licenses

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

Reagents Supplied

Application Features

Materials Required but not Supplied

NEBNext Product Citations Search Tool

Specifications & Change Notifications

Licenses

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NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®

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NEBNext^® Poly(A) mRNA Magnetic Isolation Module

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NEBNext^® Ultra™ RNA Library Prep Kit for Illumina^®