NEBNext® Poly(A) mRNA Magnetic Isolation Module
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.
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Product Information
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext RNA Binding Buffer (2X) 4 2X NEBNext Wash Buffer 4 Nuclease-free Water -20 NEBNext Tris Buffer 4 NEBNext Oligo d(T)25 Beads 4 - Product Categories:
- RNA Library Prep Products
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Protocols, Manuals & Usage
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FAQs & Troubleshooting
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