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NEBNext^® Poly(A) mRNA Magnetic Isolation Module

Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.

Catalog #	Concentration	Size
E7490S	Not Applicable	24 reactions
E7490L	Not Applicable	96 reactions
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Product Information

The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.

Reagents Supplied

The following reagents are supplied with this product:

Show all

NEB # Component Name Component # Stored at (°C) Amount Concentration

E7490S

NEBNext RNA Binding Buffer (2X)	E7492AVIAL	4	1 x 7.2 ml	Not Applicable
NEBNext Wash Buffer	E7493AVIAL	4	1 x 28.8 ml	Not Applicable
Nuclease-free Water	E7495AVIAL	4	1 x 1.2 ml	Not Applicable
NEBNext Tris Buffer	E7496AVIAL	4	1 x 6 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AVIAL	4	1 x 0.48 ml	Not Applicable

E7490L

NEBNext RNA Binding Buffer (2X)	E7492AAVIAL	4	1 x 28.8 ml	Not Applicable
NEBNext Wash Buffer	E7493AAVIAL	4	2 x 57.6 ml	Not Applicable
Nuclease-free Water	E7495AAVIAL	4	1 x 4.8 ml	Not Applicable
NEBNext Tris Buffer	E7496AAVIAL	4	1 x 24 ml	Not Applicable
NEBNext Oligo d(T)25 Beads	E7499AAVIAL	4	1 x 1.92 ml	Not Applicable

Product Categories:: RNA Library Prep Products

Applications:: RNA For Illumina,; RNA-seq,; RNA Modification,; RNA Purification and Isolation,

Advantages and Features

Application Features
Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.
Properties & Usage
Materials Required but not Supplied
- 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
- Magnetic rack or plate (e.g., NEBNext^® Magnetic Separation Rack (NEB #S1515S), Alpaqua ^® 96S Super Magnet Plate (#A001322), or equivalent)
- Thermal cycler or heat block
- Bioanalyzer^® (Agilent Technologies, Inc.) or similar instrument and consumables.

Protocols, Manuals & Usage
- Protocols
  Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)
  
  Protocol for Enrichment of mRNAs, excluding Globin mRNA, from Whole Blood Total RNA
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
  
  manualE7490
- Usage Guidelines
  Getting Started with RNA-Sequencing (RNA-Seq)
FAQs & Troubleshooting
- FAQs
  Can I isolate poly(A)⁺ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
  
  Do I need two rounds of binding?
  
  Can I use the Oligo (dT)₂₅ Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  E7490S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  E7490G_v0_10010544
  
  E7490L_v0_10012676
  
  E7490S_v0_10011311
  
  E7490G_v0_10013797
  
  E7490L_v0_10014379
  
  E7490L_v0_10016090
  
  E7490S_v0_10017723
  
  E7490L_v0_10018958
  
  E7490G_v0_10019674
  
  E7490G_v0_10021115
  
  E7490S_v0_10021149
  
  E7490L_v0_10021147
  
  E7490G_v0_10026398
  
  E7490L_v0_10025538
  
  E7490L_v0_10027982
  
  E7490S_v0_10026837
  
  E7490G_v0_10009812
  
  E7490L_v0_10030136
  
  E7490L_v0_10015484
  
  E7490S_v0_10009818
  
  E7490L_v0_10036118
  
  E7490G_v0_10037156
  
  E7490S_v0_10036204
  
  E7490L_v0_10040390
  
  E7490L_v0_10042265
  
  E7490G_v0_10041864
  
  E7490S_v0_10043249
  
  E7490G_v0_10044731
  
  E7490L_v1_10030973
  
  E7490S_v1_10046831
  
  E7490L_v1_10049111
  
  E7490G_v0_10030979
  
  E7490S_v1_10030967
  
  E7490L_v1_10050498
  
  E7490S_v1_10050322
  
  E7490G_v0_10050355
  
  E7490L_v1_10053666
  
  E7490G_v0_10054052
  
  E7490S_v1_10054050
  
  E7490L_v1_10042215
  
  E7490S_v1_10056244
  
  E7490G_v0_10042203
  
  E7490L_v1_10059821
  
  E7490S_v1_10042209
  
  E7490L_v1_10059927
  
  E7490L_v1_10061944
  
  E7490G_v0_10063351
  
  E7490L_v1_10054407
  
  E7490S_v1_10064141
  
  E7490L_v1_10067321
  
  E7490S_v1_10054401
  
  E7490G_v0_10070797
  
  E7490L_v1_10070209
  
  E7490S_v1_10071608
  
  E7490L_v1_10072375
  
  E7490L_v1_10061867
  
  E7490S_v1_10061861
  
  E7490L_v1_10080706
  
  E7490S_v1_10080973
  
  E7490L_v1_10082500
  
  E7490G_v0_10054395
  
  E7490S_v1_10084451
  
  E7490L_v1_10084857
  
  E7490G_v0_10087243
  
  E7490L_v1_10087559
  
  E7490S_v1_10087237
  
  E7490L_v1_10091923
  
  E7490S_v1_10092269
  
  E7490L_v1_10071736
  
  E7490S_v1_10071730
  
  E7490L_v1_10098045
  
  E7490G_v0_10098271
  
  E7490L_v1_10099545
  
  E7490S_v1_10100165
  
  E7490L_v1_10100885
  
  E7490L_v1_10103092
  
  E7490S_v1_10103094
  
  E7490L_v1_10103729
  
  E7490S_v1_10106917
  
  E7490G_v0_10071724
  
  E7490L_v1_10092671
  
  E7490L_v1_10110898
  
  E7490S_v1_10110353
  
  E7490L_v1_10112752
  
  E7490G_v0_10092659
  
  E7490S_v1_10092665
  
  E7490S_v1_10116719
  
  E7490L_v1_10117167
  
  E7490L_v1_10120096
  
  E7490L_v1_10121671
  
  E7490S_v1_10103517
  
  E7490L_v1_10123880
  
  E7490L_v1_10103523
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext RNA Binding Buffer (2X)
  
  NEBNext Wash Buffer
  
  Nuclease-free Water
  
  NEBNext Tris Buffer
  
  NEBNext Oligo d(T)25 Beads
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
  Licenses
  This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.

Applications

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NEBNext^® Poly(A) mRNA Magnetic Isolation Module

Reagents Supplied

Application Features

Materials Required but not Supplied

Specifications

Licenses

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NEBNext® Poly(A) mRNA Magnetic Isolation Module

Reagents Supplied

Application Features

Materials Required but not Supplied

Specifications

Licenses

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NEBNext^® Poly(A) mRNA Magnetic Isolation Module

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NEBNext^® Ultra™ RNA Library Prep Kit for Illumina^®