NEBNext^® Poly(A) mRNA Magnetic Isolation Module

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
E7490S     4       NEBNext RNA Binding Buffer (2X) E7492AVIAL 4 1 x 7.2 ml Not Applicable   NEBNext Wash Buffer E7493AVIAL 4 1 x 28.8 ml Not Applicable   Nuclease-free Water E7495AVIAL 4 1 x 1.2 ml Not Applicable   NEBNext Tris Buffer E7496AVIAL 4 1 x 6 ml Not Applicable   NEBNext Oligo d(T)25 Beads E7499AVIAL 4 1 x 0.48 ml Not Applicable
E7490L     4       NEBNext RNA Binding Buffer (2X) E7492AAVIAL 4 1 x 28.8 ml Not Applicable   NEBNext Wash Buffer E7493AAVIAL 4 2 x 57.6 ml Not Applicable   Nuclease-free Water E7495AAVIAL 4 1 x 4.8 ml Not Applicable   NEBNext Tris Buffer E7496AAVIAL 4 1 x 24 ml Not Applicable   NEBNext Oligo d(T)25 Beads E7499AAVIAL 4 1 x 1.92 ml Not Applicable

Application Features

Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.

1. Isolate mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)
2. Protocol for Enrichment of mRNAs, excluding Globin mRNA, from Whole Blood Total RNA

• Getting Started with RNA-Sequencing (RNA-Seq)

1. Can I isolate poly(A)⁺ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
2. Do I need two rounds of binding?
3. Can I use the Oligo (dT)₂₅ Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?

NEBNext Product Citations Search Tool

Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E7490S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E7490G_v0_10010544
• E7490L_v0_10012676
• E7490S_v0_10011311
• E7490G_v0_10013797
• E7490L_v0_10014379
• E7490L_v0_10016090
• E7490S_v0_10017723
• E7490L_v0_10018958
• E7490G_v0_10019674
• E7490G_v0_10021115
• E7490S_v0_10021149
• E7490L_v0_10021147
• E7490G_v0_10026398
• E7490L_v0_10025538
• E7490L_v0_10027982
• E7490S_v0_10026837
• E7490G_v0_10009812
• E7490L_v0_10030136
• E7490L_v0_10015484
• E7490S_v0_10009818
• E7490L_v0_10036118
• E7490G_v0_10037156
• E7490S_v0_10036204
• E7490L_v0_10040390
• E7490L_v0_10042265
• E7490G_v0_10041864
• E7490S_v0_10043249
• E7490G_v0_10044731
• E7490L_v1_10030973
• E7490S_v1_10046831
• E7490L_v1_10049111
• E7490G_v0_10030979
• E7490S_v1_10030967
• E7490L_v1_10050498
• E7490S_v1_10050322
• E7490G_v0_10050355
• E7490L_v1_10053666
• E7490G_v0_10054052
• E7490S_v1_10054050
• E7490L_v1_10042215
• E7490S_v1_10056244
• E7490G_v0_10042203
• E7490L_v1_10059821
• E7490S_v1_10042209
• E7490L_v1_10059927
• E7490L_v1_10061944
• E7490G_v0_10063351
• E7490L_v1_10054407
• E7490S_v1_10064141
• E7490L_v1_10067321
• E7490S_v1_10054401
• E7490G_v0_10070797
• E7490L_v1_10070209
• E7490S_v1_10071608
• E7490L_v1_10072375
• E7490L_v1_10061867
• E7490S_v1_10061861
• E7490L_v1_10080706
• E7490S_v1_10080973
• E7490L_v1_10082500
• E7490G_v0_10054395
• E7490S_v1_10084451
• E7490L_v1_10084857
• E7490G_v0_10087243
• E7490L_v1_10087559
• E7490S_v1_10087237
• E7490L_v1_10091923
• E7490S_v1_10092269
• E7490L_v1_10071736
• E7490S_v1_10071730
• E7490L_v1_10098045
• E7490G_v0_10098271
• E7490L_v1_10099545
• E7490S_v1_10100165
• E7490L_v1_10100885
• E7490L_v1_10103092
• E7490S_v1_10103094
• E7490L_v1_10103729
• E7490S_v1_10106917
• E7490G_v0_10071724
• E7490L_v1_10092671
• E7490L_v1_10110898
• E7490S_v1_10110353
• E7490L_v1_10112752
• E7490G_v0_10092659
• E7490S_v1_10092665
• E7490S_v1_10116719
• E7490L_v1_10117167
• E7490L_v1_10120096
• E7490L_v1_10121671
• E7490S_v1_10103517
• E7490L_v1_10123880
• E7490L_v1_10103523
• E7490L_v1_10129233
• E7490G_v0_10129243
• E7490L_v1_10129757
• E7490S_v1_10129439
• E7490G_v0_10131814
• E7490L_v1_10132429
• E7490S_v1_10135473
• E7490L_v1_10138378
• E7490G_v0_10141551
• E7490L_v1_10142213
• E7490S_v1_10144645
• E7490L_v1_10115769
• E7490L_v1_10151189
• E7490S_v1_10150134
• E7490S_v1_10115759
• E7490S_v1_10154850
• E7490L_v1_10155185
• E7490G_v1_10115775
• E7490G_v1_10158534
• E7490L_v1_10158066
• E7490S_v1_10158079
• E7490L_v1_10164164
• E7490S_v1_10136053
• E7490G_v1_10168729
• E7490L_v1_10169360
• E7490S_v1_10169121
• E7490L_v1_10171851

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• NEBNext RNA Binding Buffer (2X)
• NEBNext Wash Buffer
• Nuclease-free Water
• NEBNext Tris Buffer
• NEBNext Oligo d(T)25 Beads

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.