NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)
NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.
Additional NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
- High efficiency adaptor ligation
- Minimized adaptor-dimer formation
- Includes index primers for library multiplexing
- Increased sample identification specificity
Incorporating a novel hairpin loop structure, the NEBNext® Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.
NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.
Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.
Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Adaptor for Illumina -20 USER® Enzyme -20 NEBNext i501 Primer -20 NEBNext i502 Primer -20 NEBNext i503 Primer -20 NEBNext i504 Primer -20 NEBNext i505 Primer -20 NEBNext i506 Primer -20 NEBNext i507 Primer -20 NEBNext i508 Primer -20 NEBNext i701 Primer -20 NEBNext i702 Primer -20 NEBNext i703 Primer -20 NEBNext i704 Primer -20 NEBNext i705 Primer -20 NEBNext i706 Primer -20 NEBNext i707 Primer -20 NEBNext i708 Primer -20 NEBNext i709 Primer -20 NEBNext i710 Primer -20 NEBNext i711 Primer -20 NEBNext i712 Primer -20
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