NEBNext® Singleplex Oligos provide adaptors and primers to enable high yield singleplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation. The primers provided enable construction of singleplex libraries, or the universal PCR primer provided can be used in conjunction with user-supplied index primers to prepare multiplex libraries.
Note that NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.
The following reagents are supplied with this product:
| Store at (°C) | Concentration | |
| NEBNext Adaptor for Illumina | ||
| USER® Enzyme | -20 | |
| NEBNext Index 1 Primer for Illumina | ||
| NEBNext Universal PCR Primer for Illumina |
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