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Home Discontinued Products NEBNext® Singleplex Oligos for Illumina®

NEBNext® Singleplex Oligos for Illumina®

This product has been replaced by NEBNext Multiplex Oligos for Illumina. View list of products available.

Catalog # E7350 was discontinued on January 02, 2019 ;

NEBNext^® Singleplex Oligos provide adaptors and primers to enable high yield singleplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation. The primers provided enable construction of singleplex libraries, or the universal PCR primer provided can be used in conjunction with user-supplied index primers to prepare multiplex libraries.

Note that NEBNext Multiplex Oligos products are available with sets of 12- and 96- single index primers as well as dual index primers.

High efficiency adaptor ligation
Minimized adaptor-dimer formation
Suitable for singleplex library construction, or for use with user-supplied index primers

Learn more about how this product is being used in the Product Citation Tool

Product Information
Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER^® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, user-supplied barcodes can be incorporated, thereby enabling multiplexing. Note that the PCR primers supplied in the kit enable production of singleplex libraries only.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Multiplex single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact [email protected] for further information.

Figure 1: Workflow

Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
Reagents Supplied

The following reagents are supplied with this product:

Store at (°C) Concentration

NEBNext Adaptor for Illumina

USER^® Enzyme -20

NEBNext Index 1 Primer for Illumina

NEBNext Universal PCR Primer for Illumina

Product Categories:

Discontinued Products
- Properties & Usage
  Materials Required but not Supplied
  
  For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.
  
  Please Refer to the Kit Specific Protocol for using the NEBNext Singleplex Oligos for Illumina:
  
  The following kits are designed for use with the NEBNext Singleplex Oligos for Illumina:
  
  #E7645 , NEBNext Ultra II DNA Library Prep Kit for Illumina
  
  #E7595 , NEBNext Ultra II Ligation Module
  
  #E7420 , NEBNext Ultra Directional RNA Library Prep Kit for Illumina
  
  #E7530 , NEBNext Ultra RNA Library Prep Kit for Illumina
  
  #E7370 , NEBNext Ultra DNA Library Prep Kit for Illumina
  
  #E7445 , NEBNext Ultra Ligation Module
  
  #E6040 , NEBNext DNA Library Prep Master Mix Set for Illumina
  
  #E6240 , NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina
  
  #E6056 , NEBNext Quick Ligation Module
  
  #E7760 , NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
  
  #E7770, NEBNext Ultra II RNA Library Prep Kit for Illumina
  
  #E7765 , NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads
  
  #E7775 , NEBNext Ultra II RNA Library Prep with Sample Purification Beads
  
  #E7103 , NEBNext Ultra II DNA Library Prep with Sample Purification Beads
  
  #E7805 , NEBNext Ultra II FS DNA Library Prep Kit for Illumina
  
  #E6177 , NEBNext Ultra II FS DNA Library Prep Kit with Sample Purification Beads
  
  Storage Temperature
  -20°C
Protocols, Manuals & Usage
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
  
  manualE7350
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  E7350Datasheet-Lot0041307
  
  E7350Datasheet-Lot0051402
FAQs & Troubleshooting
- FAQs
  Is the Monarch Total RNA Miniprep Kit compatible with NEBNext reagents for RNA Library Prep
  
  Are NEBNext adaptors and primers for Illumina validated in Next Generation Sequencing Workflows?
  
  Are NEBNext adaptor and primers for Illumina compatible with NEBNext reagents for Illumina library preparation?
  
  Is USER enzyme required for library preparation using NEBNext adaptor and primers for Illumina?
  
  How much DNA can I ligate to the NEBNext adaptor for Illumina in one ligation reaction?
  
  Are these reagents available in a customized format or bulk format?
  
  Can USER Enzyme be purchased separately?
  
  Are the NEBNext Oligos for Illumina compatible with single read and paired end sequencing?
  
  Is the USER enzyme in our NEBNext kits identical in concentration to the standalone product?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  E7350S_L_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  E7350L_v0_10010583
  
  E7350S_v0_10010619
  
  E7350L_v1_10014342
  
  E7350S_v1_10016083
  
  E7350L_v1_10022645
  
  E7350L_v1_10025766
  
  E7350S_v1_10025501
  
  E7350L_v1_10029748
  
  E7350S_v1_10031010
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  NEBNext Adaptor for Illumina
  
  USER^® Enzyme
  
  NEBNext Index 1 Primer for Illumina
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  E7350Datasheet-Lot0041307
  
  E7350Datasheet-Lot0051402
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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