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Restriction Endonucleases

Cut Smarter with Restriction Enzymes from NEB

With over 40 years of offering restriction enzymes to the research community, NEB has earned the reputation of being a leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled performance.

All of NEB's Restriction enzymes have transitioned to a new buffer system. Visit NEBCutSmart.com for further details. 

Convenience

  • A vial of 6X Purple Load Dye is included with most restriction enzymes.
  • Over 210 restriction enzymes are 100% active in a single buffer – CutSmart™ Buffer. 
  • >190 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight.
  • Choose from >280 restriction enzymes, the largest selection commercially available.



Performance

  • Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer. A vial of 6X Purple Load Dye is included with every HF restriction enzyme.
  • All of our restriction enzymes undergo stringent quality control testing, ensuring the highest levels of purity and lot-to-lot consistency.


Use Enzyme Finder to select restriction enzymes by name, sequence, overhang or type.

Restriction Endonucleases

Restriction Endonucleases: A

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

Restriction Endonucleases: B

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFαI

BsrGI

BsrGI-HF®

BsrI

BssHII

BssSαI

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I

BstZ17I-HF®

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsαI

Restriction Endonucleases: C-G

Cac8I

ClaI

CspCI

CviAII

CviKI-1

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

Restriction Endonucleases: H-M

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188III

Hpy99I

HpyAV

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI

KpnI-HF®

LpnPI

MboI

MboII

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Restriction Endonucleases: N-O

NaeI

NarI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Restriction Endonucleases: P-R

PacI

PaeR7I

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PluTI

PmeI

PpuMI

PshAI

PsiI

PspGI

PspOMI

PspXI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

RsrII

Restriction Endonucleases: S

SacI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

Restriction Endonucleases: T-Z

TaqαI

TfiI

TseI

Tsp45I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AhdI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BbsI

BbsI-HF®

BbvI

BciVI

BclI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BlpI

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaAI

BsaHI

BsaI-HF®

BsaWI

BsaXI

BseRI

BsgI

BsiEI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmI

BsoBI

Bsp1286I

BspCNI

BspEI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrGI-HF®

BsrI

BssHII

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CspCI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI

EagI-HF®

EarI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Fnu4HI

FseI

FspEI

FspI

HaeIII

HhaI

HincII

HindIII-HF®

HinfI

HinP1I

HpaII

HphI

Hpy166II

HpyAV

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KpnI

KpnI-HF®

LpnPI

MboI

McrBC

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI

NheI-HF®

NlaIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

PacI

PaeR7I

PflFI

PflMI

PI-PspI

PI-SceI

PmeI

PpuMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

SacI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

SbfI

SbfI-HF®

ScaI-HF®

SfiI

SfoI

SmaI

SpeI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqαI

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XhoI

XmaI

XmnI

Restriction Endonuclease Buffers & Diluents

BSA, Molecular Biology Grade

CutSmart® Buffer

Diluent A

Diluent B

Diluent C

NEBuffer™ 1

NEBuffer™ 1.1

NEBuffer™ 2

NEBuffer™ 2.1

NEBuffer™ 3

NEBuffer™ 3.1

NEBuffer™ 4

NEBuffer™ EcoRI

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

S-adenosylmethionine (SAM)

Restriction Endonucleases: A

AatII

AbaSI

Acc65I

AccI

AciI

AclI

AcuI

AfeI

AflII

AflIII

AgeI

AgeI-HF®

AhdI

AleI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI-HF®

AscI

AseI

AsiSI

AvaI

AvaII

AvrII

Restriction Endonucleases: B

BaeGI

BaeI

BamHI

BamHI-HF®

BanI

BanII

BbsI

BbsI-HF®

BbvCI

BbvI

BccI

BceAI

BcgI

BciVI

BclI

BclI-HF

BcoDI

BfaI

BfuAI

BglI

BglII

BlpI

BmgBI

BmrI

BmtI

BmtI-HF®

BpmI

Bpu10I

BpuEI

BsaAI

BsaBI

BsaHI

BsaI

BsaI-HF®

BsaJI

BsaWI

BsaXI

BseRI

BseYI

BsgI

BsiEI

BsiHKAI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmFI

BsmI

BsoBI

Bsp1286I

BspCNI

BspDI

BspEI

BspHI

BspMI

BspQI

BsrBI

BsrDI

BsrFI

BsrFαI

BsrGI

BsrGI-HF®

BsrI

BssHII

BssSαI

BstAPI

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I

BstZ17I-HF®

Bsu36I

BtgI

BtgZI

BtsCI

BtsIMutI

BtsαI

Restriction Endonucleases: C-G

Cac8I

ClaI

CspCI

CviAII

CviKI-1

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EaeI

EagI

EagI-HF®

EarI

EciI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

FatI

FauI

Fnu4HI

FokI

FseI

FspEI

FspI

Restriction Endonucleases: H-M

HaeII

HaeIII

HgaI

HhaI

HincII

HindIII

HindIII-HF®

HinfI

HinP1I

HpaI

HpaII

HphI

Hpy166II

Hpy188III

Hpy99I

HpyAV

HpyCH4III

HpyCH4IV

HpyCH4V

I-CeuI

I-SceI

KasI

KpnI

KpnI-HF®

LpnPI

MboI

MboII

MfeI

MfeI-HF®

MluCI

MluI

MluI-HF®

MlyI

MmeI

MnlI

MscI

MseI

MslI

MspA1I

MspI

MspJI

MwoI

Restriction Endonucleases: N-O

NaeI

NarI

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI

NheI-HF®

NlaIII

NlaIV

NmeAIII

NotI

NotI-HF®

NruI

NruI-HF®

NsiI

NsiI-HF®

NspI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Restriction Endonucleases: P-R

PacI

PaeR7I

PciI

PflFI

PflMI

PI-PspI

PI-SceI

PleI

PluTI

PmeI

PpuMI

PshAI

PsiI

PspGI

PspOMI

PspXI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

RsrII

Restriction Endonucleases: S

SacI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

Sau3AI

Sau96I

SbfI

SbfI-HF®

ScaI-HF®

ScrFI

SexAI

SfaNI

SfcI

SfiI

SfoI

SgrAI

SmaI

SmlI

SnaBI

SpeI

SpeI-HF®

SphI

SphI-HF®

SrfI

SspI

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

Restriction Endonucleases: T-Z

TaqαI

TfiI

TseI

Tsp45I

TspMI

TspRI

Tth111I

XbaI

XcmI

XhoI

XmaI

XmnI

ZraI

HF®, Nicking, Master Mix, Time-Saver & Other

High-Fidelity (HF®) Restriction Endonucleases

AgeI-HF®

ApoI-HF®

BamHI-HF®

BbsI-HF®

BclI-HF

BmtI-HF®

BsaI-HF®

BsiWI-HF®

BsrGI-HF®

BstEII-HF®

BstZ17I-HF®

DraIII-HF®

EagI-HF®

EcoRI-HF®

EcoRV-HF®

HindIII-HF®

KpnI-HF®

MfeI-HF®

MluI-HF®

NcoI-HF®

NheI-HF®

NotI-HF®

NruI-HF®

NsiI-HF®

PstI-HF®

PvuI-HF®

PvuII-HF®

SacI-HF®

SalI-HF®

SbfI-HF®

ScaI-HF®

SpeI-HF®

SphI-HF®

SspI-HF®

StyI-HF®

Homing Endonucleases

I-CeuI

I-SceI

PI-PspI

PI-SceI

Nicking Endonucleases

Nb.BbvCI

Nb.BsmI

Nb.BsrDI

Nb.BssSI

Nb.BtsI

Nt.AlwI

Nt.BbvCI

Nt.BsmAI

Nt.BspQI

Nt.BstNBI

Restriction Enzymes for Epigenetic Analysis (EpiMark® Validated)

AbaSI

DpnI

DpnII

FspEI

HpaII

LpnPI

McrBC

MspI

MspJI

Time-Saver™ Qualified Restriction Enzymes

AatII

Acc65I

AccI

AciI

AclI

AcuI

AflII

AgeI-HF®

AhdI

AluI

AlwNI

ApaI

ApaLI

ApeKI

ApoI-HF®

AscI

AseI

AvaI

AvaII

AvrII

BaeGI

BaeI

BamHI

BamHI-HF®

BbsI

BbsI-HF®

BbvI

BciVI

BclI

BclI-HF

BcoDI

BfuAI

BglI

BglII

BlpI

BmgBI

BmtI-HF®

BpmI

BpuEI

BsaAI

BsaHI

BsaI-HF®

BsaWI

BsaXI

BseRI

BsgI

BsiEI

BsiWI

BsiWI-HF®

BslI

BsmAI

BsmBI

BsmI

BsoBI

Bsp1286I

BspCNI

BspEI

BspHI

BspQI

BsrBI

BsrDI

BsrGI

BsrI

BssHII

BstBI

BstEII

BstEII-HF®

BstNI

BstUI

BstXI

BstYI

BstZ17I

BstZ17I-HF®

Bsu36I

BtgI

BtsCI

Cac8I

ClaI

CspCI

CviAII

CviQI

DdeI

DpnI

DpnII

DraI

DraIII-HF®

DrdI

EagI

EagI-HF®

EarI

Eco53kI

EcoNI

EcoO109I

EcoP15I

EcoRI

EcoRI-HF®

EcoRV

EcoRV-HF®

Fnu4HI

FseI

FspI

HaeIII

HhaI

HincII

HindIII-HF®

HinfI

HinP1I

HpaII

HphI

Hpy166II

HpyAV

HpyCH4IV

HpyCH4V

KpnI

KpnI-HF®

MboI

MfeI-HF®

MluCI

MluI

MlyI

MmeI

MnlI

MseI

MslI

MspA1I

MspI

MwoI

NciI

NcoI

NcoI-HF®

NdeI

NgoMIV

NheI

NheI-HF®

NlaIII

NotI

NotI-HF®

NruI

NsiI

NspI

PacI

PaeR7I

PflFI

PflMI

PmeI

PpuMI

PshAI

PstI

PstI-HF®

PvuI

PvuI-HF®

PvuII

PvuII-HF®

RsaI

SacI

SacI-HF®

SacII

SalI

SalI-HF®

SapI

SbfI

SbfI-HF®

ScaI-HF®

SfiI

SfoI

SmaI

SpeI

SpeI-HF®

SphI-HF®

SspI-HF®

StuI

StyD4I

StyI

StyI-HF®

SwaI

TaqαI

TfiI

TseI

TspMI

TspRI

Tth111I

XbaI

XhoI

XmaI

XmnI

Restriction Endonuclease Buffers & Diluents

BSA, Molecular Biology Grade

CutSmart® Buffer

Diluent A

Diluent B

Diluent C

NEBuffer™ 1

NEBuffer™ 1.1

NEBuffer™ 2

NEBuffer™ 2.1

NEBuffer™ 3

NEBuffer™ 3.1

NEBuffer™ 4

NEBuffer™ EcoRI

NEBuffer™ Set (1.1, 2.1, 3.1 and CutSmart®)

S-adenosylmethionine (SAM)

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