NEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single buffer, CutSmart® . This improves ease-of-use, especially when performing double digests. In addition to indicating the performance of each enzyme in the 4 NEBuffers, the chart also indicates ligation and recutting, star activity, and whether or not more than 1-site is required for cleavage.
Should you require information on the pre-2013 buffer system, please refer to the old NEBuffer Activity Chart. You can also receive additional support by contacting [email protected].
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Not Sensitive | ![]() |
Impaired |
---|---|---|---|
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Blocked | ![]() |
Impaired by Overlapping |
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Blocked by Overlapping | ![]() |
Impaired by Some Combinations of Overlapping |
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Blocked by Some Combinations of Overlapping |
Please check out other technical reference information related to restriction enzymes:
Double Digestion | Heat Inactivation | Activity at 37°C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
AatII | ![]() ![]() ![]() |
GACGT/C | CutSmart® Buffer | <10 | 50* | 50 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
AbaSI | ![]() |
CNNNNNNNNNNN/NNNNNNNNNG | CutSmart® Buffer | 25 | 50 | 50 | 100 | 65°C | 25°C | C | ![]() |
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T4 wild-type phage DNA (fully ghmC-modified) | e |
Acc65I | ![]() ![]() ![]() ![]() |
G/GTACC | NEBuffer™ 3.1 | 10 | 75* | 100 | 25 | 65°C | 37°C | A | ![]() |
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pBC4 DNA | |
AccI | ![]() ![]() ![]() |
GT/MKAC | CutSmart® Buffer | 50 | 50 | 10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
AciI | ![]() ![]() ![]() |
CCGC(-3/-1) | CutSmart® Buffer | <10 | 25 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
AclI | ![]() ![]() ![]() |
AA/CGTT | CutSmart® Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | ![]() |
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λ DNA | |
AcuI | ![]() ![]() |
CTGAAG(16/14) | CutSmart® Buffer + SAM | 50 | 100 | 50 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | 1, b, d |
AfeI | ![]() ![]() |
AGC/GCT | CutSmart® Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | |
AflII | ![]() ![]() |
C/TTAAG | CutSmart® Buffer | 50 | 100 | 10 | 100 | 65°C | 37°C | A | ![]() |
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ΦX174 RF I DNA | |
AflIII | ![]() |
A/CRYGT | NEBuffer™ 3.1 | 10 | 50 | 100 | 50 | 80°C | 37°C | B | ![]() |
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λ DNA | |
AgeI § | ![]() ![]() |
A/CCGGT | NEBuffer™ 1.1 | 100 | 75 | 25 | 75 | 65°C | 37°C | C | ![]() |
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λ DNA | |
AgeI-HF® | ![]() ![]() ![]() ![]() |
A/CCGGT | CutSmart® Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
AhdI | ![]() ![]() ![]() |
GACNNN/NNGTC | CutSmart® Buffer | 25 | 25 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | a |
AleI-v2 | ![]() ![]() ![]() |
CACNN/NNGTG | CutSmart® Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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AluI | ![]() ![]() |
AG/CT | CutSmart® Buffer | 25 | 100 | 50 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | b |
AlwI | ![]() ![]() |
GGATC(4/5) | CutSmart® Buffer | 50 | 50 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA (dam-) | 1, b, d |
AlwNI | ![]() ![]() ![]() |
CAGNNN/CTG | CutSmart® Buffer | 10 | 100 | 50 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
ApaI | ![]() ![]() ![]() ![]() |
GGGCC/C | CutSmart® Buffer | 25 | 25 | <10 | 100 | 65°C | 25°C | A | ![]() |
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pXba DNA | |
ApaLI | ![]() ![]() ![]() |
G/TGCAC | CutSmart® Buffer | 100 | 100 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
ApeKI | ![]() ![]() ![]() |
G/CWGC | NEBuffer™ 3.1 | 25 | 50 | 100 | 10 | No | 75°C | B | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
ApoI § | ![]() ![]() |
R/AATTY | NEBuffer™ 3.1 | 10 | 75 | 100 | 75 | 80°C | 50°C | A | ![]() |
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λ DNA | |
ApoI-HF | ![]() ![]() ![]() |
R/AATTY | CutSmart® Buffer | 10 | 100 | 10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
AscI | ![]() ![]() ![]() |
GG/CGCGCC | CutSmart® Buffer | <10 | 10 | 10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
AseI | ![]() ![]() |
AT/TAAT | NEBuffer™ 3.1 | <10 | 50* | 100 | 10 | 65°C | 37°C | B | ![]() |
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λ DNA | 3 |
AsiSI | ![]() ![]() |
GCGAT/CGC | CutSmart® Buffer | 100 | 100 | 25 | 100 | 80°C | 37°C | B | ![]() |
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XhoI digested pXba | 2, b |
AvaI | ![]() ![]() ![]() |
C/YCGRG | CutSmart® Buffer | <10 | 100 | 25 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
AvaII | ![]() ![]() ![]() ![]() |
G/GWCC | CutSmart® Buffer | 50 | 75 | 10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
AvrII | ![]() ![]() |
C/CTAGG | CutSmart® Buffer | 100 | 50 | 50 | 100 | No | 37°C | B | ![]() |
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λ DNA (HindIII digest) | |
BaeGI | ![]() ![]() |
GKGCM/C | NEBuffer™ 3.1 | 75 | 75 | 100 | 25 | 80°C | 37°C | A | ![]() |
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λ DNA | |
BaeI | ![]() ![]() ![]() |
(10/15)ACNNNNGTAYC(12/7) | CutSmart® Buffer + SAM | 50 | 100 | 50 | 100 | 65°C | 25°C | A | ![]() |
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λ DNA | e |
BamHI § | ![]() ![]() |
G/GATCC | NEBuffer™ 3.1 | 75* | 100* | 100 | 100* | No | 37°C | A | ![]() |
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λ DNA | 3 |
BamHI-HF® | ![]() ![]() ![]() |
G/GATCC | CutSmart® Buffer | 100 | 50 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
BanI | ![]() ![]() ![]() |
G/GYRCC | CutSmart® Buffer | 10 | 25 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | 1 |
BanII | ![]() |
GRGCY/C | CutSmart® Buffer | 100 | 100 | 50 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | 2 |
BbsI § | ![]() ![]() |
GAAGAC(2/6) | NEBuffer™ 2.1 | 100 | 100 | 25 | 75 | 65°C | 37°C | B | ![]() |
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λ DNA | |
BbsI-HF® | ![]() ![]() ![]() |
GAAGAC(2/6) | CutSmart® Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
BbvCI | ![]() ![]() |
CCTCAGC(-5/-2) | CutSmart® Buffer | 10 | 100 | 50 | 100 | No | 37°C | B | ![]() |
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λ DNA | 1, a |
BbvI | ![]() ![]() |
GCAGC(8/12) | CutSmart® Buffer | 100 | 100 | 25 | 100 | 65°C | 37°C | B | ![]() |
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pBR322 DNA | 3 |
BccI | ![]() |
CCATC(4/5) | CutSmart® Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | 3, b |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
BceAI | ![]() ![]() |
ACGGC(12/14) | NEBuffer™ 3.1 | 100* | 100* | 100 | 100* | 65°C | 37°C | A | ![]() |
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pBR322 DNA | 1 |
BcgI | ![]() ![]() ![]() |
(10/12)CGANNNNNNTGC(12/10) | NEBuffer™ 3.1 + SAM | 10 | 75* | 100 | 50* | 65°C | 37°C | A | ![]() |
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λ DNA | e |
BciVI | ![]() ![]() |
GTATCC(6/5) | CutSmart® Buffer | 100 | 25 | <10 | 100 | 80°C | 37°C | C | ![]() |
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λ DNA | b |
BclI § | ![]() ![]() ![]() |
T/GATCA | NEBuffer™ 3.1 | 50 | 100 | 100 | 75 | No | 50°C | A | ![]() |
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λ DNA (dam-) | |
BclI-HF | ![]() ![]() ![]() ![]() |
T/GATCA | CutSmart® Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA (dam-) | |
BcoDI | ![]() ![]() ![]() |
GTCTC(1/5) | CutSmart® Buffer | 50 | 75 | 75 | 100 | No | 37°C | B | ![]() |
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λ DNA | |
BfaI | ![]() |
C/TAG | CutSmart® Buffer | <10 | 10 | <10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | 2, b |
BfuAI | ![]() ![]() ![]() |
ACCTGC(4/8) | NEBuffer™ 3.1 | <10 | 25 | 100 | 10 | 65°C | 50°C | B | ![]() |
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λ DNA | 3 |
BglI | ![]() ![]() ![]() |
GCCNNNN/NGGC | NEBuffer™ 3.1 | 10 | 25 | 100 | 10 | 65°C | 37°C | B | ![]() |
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λ DNA | |
BglII | ![]() ![]() |
A/GATCT | NEBuffer™ 3.1 | 10 | 10 | 100 | <10 | No | 37°C | A | ![]() |
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λ DNA | |
BlpI | ![]() ![]() |
GC/TNAGC | CutSmart® Buffer | 50 | 100 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | d |
BmgBI | ![]() ![]() ![]() |
CACGTC(-3/-3) | NEBuffer™ 3.1 | <10 | 10 | 100 | 10 | 65°C | 37°C | B | ![]() |
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λ DNA | 3, b, d |
BmrI | ![]() |
ACTGGG(5/4) | NEBuffer™ 2.1 | 75 | 100 | 75 | 100* | 65°C | 37°C | B | ![]() |
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λ DNA (HindIII digest) | b |
BmtI § | ![]() |
GCTAG/C | NEBuffer™ 3.1 | 100 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | 2 |
BmtI-HF® | ![]() ![]() ![]() |
GCTAG/C | CutSmart® Buffer | 50 | 100 | 10 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | |
BpmI | ![]() |
CTGGAG(16/14) | NEBuffer™ 3.1 | 75 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | 2 |
BpuEI | ![]() ![]() |
CTTGAG(16/14) | CutSmart® Buffer + SAM | 50* | 100 | 50* | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | d |
Bpu10I | ![]() |
CCTNAGC(-5/-2) | NEBuffer™ 3.1 | 10 | 25 | 100 | 25 | 80°C | 37°C | B | ![]() |
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λ DNA | 3, b, d |
BsaAI | ![]() ![]() ![]() |
YAC/GTR | CutSmart® Buffer | 100 | 100 | 100 | 100 | No | 37°C | C | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
BsaBI | ![]() ![]() ![]() |
GATNN/NNATC | CutSmart® Buffer | 50 | 100 | 75 | 100 | 80°C | 60°C | B | ![]() |
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λ DNA (dam-) | 2 |
BsaHI | ![]() ![]() ![]() ![]() |
GR/CGYC | CutSmart® Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | C | ![]() |
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λ DNA | |
BsaI § | ![]() ![]() ![]() |
GGTCTC(1/5) | CutSmart® Buffer | 75* | 75 | 100 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | 3 |
BsaI-HF®v2 | ![]() ![]() ![]() ![]() ![]() |
GGTCTC(1/5) | CutSmart® Buffer | 100 | 100 | 100 | 100 | 80°C | 37°C | B | ![]() |
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pXba DNA | |
BsaJI | ![]() |
C/CNNGG | CutSmart® Buffer | 50 | 100 | 100 | 100 | 80°C | 60°C | A | ![]() |
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λ DNA | |
BsaWI | ![]() ![]() |
W/CCGGW | CutSmart® Buffer | 10 | 100 | 50 | 100 | 80°C | 60°C | A | ![]() |
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λ DNA | |
BsaXI | ![]() |
(9/12)ACNNNNNCTCC(10/7) | CutSmart® Buffer | 50* | 100* | 10 | 100 | No | 37°C | C | ![]() |
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λ DNA | e |
BseRI | ![]() ![]() |
GAGGAG(10/8) | CutSmart® Buffer | 100 | 100 | 75 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | d |
BseYI | ![]() ![]() |
CCCAGC(-5/-1) | NEBuffer™ 3.1 | 10 | 50 | 100 | 50 | 80°C | 37°C | B | ![]() |
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λ DNA | d |
BsgI | ![]() ![]() |
GTGCAG(16/14) | CutSmart® Buffer + SAM | 25 | 50 | 25 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | d |
BsiEI | ![]() ![]() ![]() |
CGRY/CG | CutSmart® Buffer | 25 | 50 | <10 | 100 | No | 60°C | A | ![]() |
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λ DNA | |
BsiHKAI | ![]() ![]() |
GWGCW/C | CutSmart® Buffer | 25 | 100 | 100 | 100 | No | 65°C | A | ![]() |
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λ DNA | |
BsiWI § | ![]() ![]() ![]() |
C/GTACG | NEBuffer™ 3.1 | 25 | 50* | 100 | 25 | 65°C | 55°C | B | ![]() |
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ΦX174 DNA | |
BsiWI-HF® | ![]() ![]() ![]() ![]() |
C/GTACG | CutSmart® Buffer | 50 | 100 | 10 | 100 | No | 37°C | B | ![]() |
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ΦX174 DNA | |
BslI | ![]() ![]() ![]() ![]() |
CCNNNNN/NNGG | CutSmart® Buffer | 50 | 75 | 100 | 100 | No | 55°C | A | ![]() |
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λ DNA | b |
BsmAI | ![]() ![]() ![]() |
GTCTC(1/5) | CutSmart® Buffer | 50 | 100 | 100 | 100 | No | 55°C | B | ![]() |
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λ DNA | |
BsmBI | ![]() ![]() ![]() |
CGTCTC(1/5) | NEBuffer™ 3.1 | 10 | 50* | 100 | 25 | 80°C | 55°C | B | ![]() |
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λ DNA | |
BsmFI | ![]() ![]() ![]() |
GGGAC(10/14) | CutSmart® Buffer | 25 | 50 | 50 | 100 | 80°C | 65°C | A | ![]() |
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pBR322 DNA | 1 |
BsmI | ![]() ![]() |
GAATGC(1/-1) | CutSmart® Buffer | 25 | 100 | <10 | 100 | 80°C | 65°C | A | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
BsoBI | ![]() ![]() |
C/YCGRG | CutSmart® Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
BspCNI | ![]() ![]() |
CTCAG(9/7) | CutSmart® Buffer + SAM | 100 | 75 | 10 | 100 | 80°C | 25°C | A | ![]() |
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λ DNA | b |
BspDI | ![]() ![]() ![]() |
AT/CGAT | CutSmart® Buffer | 25 | 75 | 50 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
BspEI | ![]() ![]() ![]() ![]() |
T/CCGGA | NEBuffer™ 3.1 | <10 | 10 | 100 | <10 | 80°C | 37°C | B | ![]() |
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λ DNA (dam-) | |
BspHI | ![]() ![]() ![]() |
T/CATGA | CutSmart® Buffer | 10 | 50 | 25 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
Bsp1286I | ![]() ![]() |
GDGCH/C | CutSmart® Buffer | 25 | 25 | 25 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | 3 |
BspMI | ![]() |
ACCTGC(4/8) | NEBuffer™ 3.1 | 10 | 50* | 100 | 10 | 65°C | 37°C | B | ![]() |
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λ DNA | |
BspQI | ![]() ![]() |
GCTCTTC(1/4) | NEBuffer™ 3.1 | 100* | 100* | 100 | 100* | 80°C | 50°C | B | ![]() |
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λ DNA | 3 |
BsrBI | ![]() ![]() ![]() |
CCGCTC(-3/-3) | CutSmart® Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | d |
BsrDI | ![]() ![]() |
GCAATG(2/0) | NEBuffer™ 2.1 | 10 | 100 | 75 | 25 | 80°C | 65°C | A | ![]() |
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λ DNA | 3, d |
BsrFI-v2 | ![]() ![]() ![]() ![]() |
R/CCGGY | CutSmart® Buffer | 25 | 25 | 0 | 100 | No | 37°C | C | ![]() |
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pBR322 DNA | |
BsrGI § | ![]() ![]() |
T/GTACA | NEBuffer™ 2.1 | 25 | 100 | 100 | 25 | 80°C | 37°C | A | ![]() |
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λ DNA | |
BsrGI-HF® | ![]() ![]() ![]() |
T/GTACA | CutSmart® Buffer | 10 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
BsrI | ![]() |
ACTGG(1/-1) | NEBuffer™ 3.1 | <10 | 50 | 100 | 10 | 80°C | 65°C | B | ![]() |
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ΦX174 DNA | b |
BssHII | ![]() ![]() ![]() |
G/CGCGC | CutSmart® Buffer | 100 | 100 | 100 | 100 | 65°C | 50°C | B | ![]() |
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λ DNA | |
BssSI-v2 | ![]() ![]() ![]() |
CACGAG(-5/-1) | CutSmart® Buffer | 10 | 25 | <10 | 100 | No | 37°C | B | ![]() |
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λ DNA | |
BstAPI | ![]() ![]() |
GCANNNN/NTGC | CutSmart® Buffer | 50 | 100 | 25 | 100 | 80°C | 60°C | A | ![]() |
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λ DNA | b |
BstBI | ![]() ![]() ![]() |
TT/CGAA | CutSmart® Buffer | 75 | 100 | 10 | 100 | No | 65°C | A | ![]() |
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λ DNA | |
BstEII § | ![]() ![]() |
G/GTNACC | NEBuffer™ 3.1 | 10 | 75* | 100 | 75* | No | 60°C | A | ![]() |
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λ DNA | 3 |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
BstEII-HF® | ![]() ![]() ![]() |
G/GTNACC | CutSmart® Buffer | <10 | 10 | <10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
BstNI | ![]() ![]() |
CC/WGG | NEBuffer™ 3.1 | 10 | 100 | 100 | 75 | No | 60°C | A | ![]() |
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λ DNA | a |
BstUI | ![]() ![]() |
CG/CG | CutSmart® Buffer | 50 | 100 | 25 | 100 | No | 60°C | A | ![]() |
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λ DNA | b |
BstXI | ![]() ![]() ![]() |
CCANNNNN/NTGG | NEBuffer™ 3.1 | <10 | 50 | 100 | 25 | 80°C | 37°C | B | ![]() |
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λ DNA | 3 |
BstYI | ![]() ![]() |
R/GATCY | NEBuffer™ 2.1 | 25 | 100 | 75 | 100 | No | 60°C | A | ![]() |
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λ DNA | |
BstZ17I-HF® | ![]() ![]() ![]() ![]() |
GTATAC | CutSmart® Buffer | 100 | 100 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
Bsu36I | ![]() ![]() |
CC/TNAGG | CutSmart® Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | C | ![]() |
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λ DNA (HindIII digest) | b |
BtgI | ![]() ![]() |
C/CRYGG | CutSmart® Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | B | ![]() |
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pBR322 DNA | |
BtgZI | ![]() ![]() |
GCGATG(10/14) | CutSmart® Buffer | 10 | 25 | <10 | 100 | 80°C | 60°C | A | ![]() |
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λ DNA | 3, b, d |
BtsCI | ![]() ![]() |
GGATG(2/0) | CutSmart® Buffer | 10 | 100 | 25 | 100 | 80°C | 50°C | B | ![]() |
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λ DNA | |
BtsIMutI | ![]() ![]() |
CAGTG(2/0) | CutSmart® Buffer | 100 | 50 | 10 | 100 | 80°C | 55°C | A | ![]() |
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pUC19 DNA | b |
BtsI-v2 | ![]() ![]() ![]() |
GCAGTG(2/0) | CutSmart® Buffer | 100 | 100 | 25 | 100 | No | 55°C | A | ![]() |
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λ DNA | |
Cac8I | ![]() ![]() |
GCN/NGC | CutSmart® Buffer | 50 | 75 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | b |
ClaI | ![]() ![]() ![]() ![]() |
AT/CGAT | CutSmart® Buffer | 10 | 50 | 50 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (dam-) | |
CspCI | ![]() ![]() |
(11/13)CAANNNNNGTGG(12/10) | CutSmart® Buffer + SAM | 10 | 100 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | e |
CviAII | ![]() ![]() |
C/ATG | CutSmart® Buffer | 50 | 50 | 10 | 100 | 65°C | 25°C | C | ![]() |
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λ DNA | |
CviKI-1 | ![]() |
RG/CY | CutSmart® Buffer | 25 | 100 | 100 | 100 | No | 37°C | A | ![]() |
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pBR322 DNA | 1, b |
CviQI | ![]() ![]() |
G/TAC | NEBuffer™ 3.1 | 75 | 100* | 100 | 75* | No | 25°C | C | ![]() |
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λ DNA | b |
DdeI | ![]() ![]() |
C/TNAG | CutSmart® Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
DpnI | ![]() ![]() ![]() |
GA/TC | CutSmart® Buffer | 100 | 100 | 75 | 100 | 80°C | 37°C | B | ![]() |
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pBR322 DNA (dam methylated) | b |
DpnII | ![]() ![]() ![]() |
/GATC | NEBuffer™ DpnII | 25 | 25 | 100* | 25 | 65°C | 37°C | B | ![]() |
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λ DNA (dam-) | |
DraI | ![]() ![]() |
TTT/AAA | CutSmart® Buffer | 75 | 75 | 50 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
DraIII-HF® | ![]() ![]() ![]() ![]() |
CACNNN/GTG | CutSmart® Buffer | <10 | 50 | 10 | 100 | No | 37°C | B | ![]() |
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λ DNA | b |
DrdI | ![]() ![]() |
GACNNNN/NNGTC | CutSmart® Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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pUC19 DNA | 3 |
EaeI | ![]() ![]() ![]() |
Y/GGCCR | CutSmart® Buffer | 10 | 50 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
EagI § | ![]() ![]() ![]() |
C/GGCCG | NEBuffer™ 3.1 | 10 | 25 | 100 | 10 | 65°C | 37°C | B | ![]() |
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pXba DNA | |
EagI-HF® | ![]() ![]() ![]() ![]() |
C/GGCCG | CutSmart® Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | |
EarI | ![]() ![]() ![]() |
CTCTTC(1/4) | CutSmart® Buffer | 50 | 10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | b, d |
EciI | ![]() ![]() |
GGCGGA(11/9) | CutSmart® Buffer | 100 | 50 | 50 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | 2 |
Eco53kI | ![]() ![]() ![]() |
GAG/CTC | CutSmart® Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | 3, b |
EcoNI | ![]() ![]() |
CCTNN/NNNAGG | CutSmart® Buffer | 50 | 100 | 75 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
EcoO109I | ![]() ![]() ![]() |
RG/GNCCY | CutSmart® Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | 3 |
EcoP15I | ![]() ![]() |
CAGCAG(25/27) | NEBuffer™ 3.1 + ATP | 75 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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pUC19 DNA | e |
EcoRI § | ![]() ![]() ![]() |
G/AATTC | NEBuffer™ EcoRI | 25 | 100* | 50 | 50* | 65°C | 37°C | C | ![]() |
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λ DNA | |
EcoRI-HF® | ![]() ![]() ![]() ![]() |
G/AATTC | CutSmart® Buffer | 10 | 100 | <10 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA | |
EcoRV § | ![]() ![]() ![]() |
GAT/ATC | NEBuffer™ 3.1 | 10 | 50 | 100 | 10 | 80°C | 37°C | A | ![]() |
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λ DNA | |
EcoRV-HF® | ![]() ![]() ![]() ![]() |
GAT/ATC | CutSmart® Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
Esp3I | ![]() ![]() ![]() |
CGTCTC(1/5) | CutSmart® Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
FatI | ![]() |
/CATG | NEBuffer™ 2.1 | 10 | 100 | 50 | 50 | 80°C | 55°C | A | ![]() |
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pUC19 DNA | |
FauI | ![]() ![]() |
CCCGC(4/6) | CutSmart® Buffer | 100 | 50 | 10 | 100 | 65°C | 55°C | A | ![]() |
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λ DNA | 3, b, d |
Fnu4HI | ![]() ![]() ![]() |
GC/NGC | CutSmart® Buffer | <10 | <10 | <10 | 100 | No | 37°C | A | ![]() |
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λ DNA | a |
FokI | ![]() ![]() ![]() |
GGATG(9/13) | CutSmart® Buffer | 100 | 100 | 75 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | 3, b, d |
FseI | ![]() ![]() ![]() ![]() |
GGCCGG/CC | CutSmart® Buffer | 100 | 75 | <10 | 100 | 65°C | 37°C | B | ![]() |
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pBC4 DNA | |
FspEI | ![]() |
CC(12/16) | CutSmart® Buffer | <10 | <10 | <10 | 100 | 80°C | 37°C | B | ![]() |
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pBR322 (dcm+) DNA | 1, e |
FspI | ![]() ![]() ![]() |
TGC/GCA | CutSmart® Buffer | 10 | 100 | 10 | 100 | No | 37°C | C | ![]() |
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λ DNA | b |
HaeII | ![]() ![]() ![]() |
RGCGC/Y | CutSmart® Buffer | 25 | 100 | 10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
HaeIII | ![]() ![]() |
GG/CC | CutSmart® Buffer | 50 | 100 | 25 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
HgaI | ![]() ![]() |
GACGC(5/10) | NEBuffer™ 1.1 | 100 | 100 | 25 | 100 | 65°C | 37°C | A | ![]() |
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ΦX174 DNA | 1 |
HhaI | ![]() ![]() ![]() |
GCG/C | CutSmart® Buffer | 25 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
HincII | ![]() ![]() ![]() |
GTY/RAC | NEBuffer™ 3.1 | 25 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
HindIII § | ![]() |
A/AGCTT | NEBuffer™ 2.1 | 25 | 100 | 50 | 50 | 80°C | 37°C | B | ![]() |
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λ DNA | 2 |
HindIII-HF® | ![]() ![]() ![]() |
A/AGCTT | CutSmart® Buffer | 10 | 100 | 10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
HinfI | ![]() ![]() ![]() |
G/ANTC | CutSmart® Buffer | 50 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
HinP1I | ![]() ![]() ![]() |
G/CGC | CutSmart® Buffer | 100 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
HpaI | ![]() ![]() |
GTT/AAC | CutSmart® Buffer | <10 | 75* | 25 | 100 | No | 37°C | A | ![]() |
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λ DNA | 1 |
HpaII | ![]() ![]() ![]() |
C/CGG | CutSmart® Buffer | 100 | 50 | <10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
HphI | ![]() ![]() ![]() ![]() |
GGTGA(8/7) | CutSmart® Buffer | 50 | 50 | <10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | b, d |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
HpyAV | ![]() ![]() ![]() |
CCTTC(6/5) | CutSmart® Buffer | 100 | 100 | 25 | 100 | 65°C | 37°C | ![]() |
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λ DNA | 3, b, d | |
HpyCH4III | ![]() |
ACN/GT | CutSmart® Buffer | 100 | 25 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
HpyCH4IV | ![]() ![]() ![]() |
A/CGT | CutSmart® Buffer | 100 | 50 | 25 | 100 | 65°C | 37°C | A | ![]() |
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pUC19 DNA | |
HpyCH4V | ![]() ![]() |
TG/CA | CutSmart® Buffer | 50 | 50 | 25 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
Hpy188I | ![]() ![]() |
TCN/GA | CutSmart® Buffer | 25 | 100 | 50 | 100 | 65°C | 37°C | A | ![]() |
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pBR322 DNA | 1, b |
Hpy99I | ![]() ![]() |
CGWCG/ | CutSmart® Buffer | 50 | 10 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
Hpy166II | ![]() ![]() ![]() |
GTN/NAC | CutSmart® Buffer | 100 | 100 | 50 | 100 | 65°C | 37°C | C | ![]() |
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pBR322 DNA | |
Hpy188III | ![]() ![]() ![]() |
TC/NNGA | CutSmart® Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | B | ![]() |
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pUC19 DNA | 3, b |
I-CeuI | ![]() |
TAACTATAACGGTCCTAAGGTAGCGAA(-9/-13) | CutSmart® Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | pBHS ScaI-linearized Control Plasmid | ||||
I-SceI | ![]() |
TAGGGATAACAGGGTAAT(-9/-13) | CutSmart® Buffer | 10 | 50 | 25 | 100 | 65°C | 37°C | B | pGPS2 NotI-linearized Control Plasmid | ||||
KasI | ![]() ![]() |
G/GCGCC | CutSmart® Buffer | 50 | 100 | 50 | 100 | 65°C | 37°C | B | ![]() |
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pBR322 DNA | 3 |
KpnI § | ![]() |
GGTAC/C | NEBuffer™ 1.1 | 100 | 75 | <10 | 50* | No | 37°C | A | ![]() |
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pXba DNA | 1 |
KpnI-HF® | ![]() ![]() ![]() |
GGTAC/C | CutSmart® Buffer | 100 | 25 | <10 | 100 | No | 37°C | A | ![]() |
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pXba DNA | |
LpnPI | ![]() |
CCDG(10/14) | CutSmart® Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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pBR322 (dcm+) DNA | 1, e |
MboI | ![]() ![]() ![]() ![]() |
/GATC | CutSmart® Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (dam-) | |
MboII | ![]() ![]() |
GAAGA(8/7) | CutSmart® Buffer | 100* | 100 | 50 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA (dam-) | b |
MfeI § | ![]() |
C/AATTG | CutSmart® Buffer | 75 | 50 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | 2 |
MfeI-HF® | ![]() ![]() ![]() |
C/AATTG | CutSmart® Buffer | 75 | 25 | <10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
MluCI | ![]() ![]() |
/AATT | CutSmart® Buffer | 100 | 10 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
MluI § | ![]() ![]() ![]() |
A/CGCGT | NEBuffer™ 3.1 | 10 | 50 | 100 | 25 | 80°C | 37°C | A | ![]() |
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λ DNA | |
MluI-HF® | ![]() ![]() ![]() ![]() |
A/CGCGT | CutSmart® Buffer | 25 | 100 | 100 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
MlyI | ![]() ![]() |
GAGTC(5/5) | CutSmart® Buffer | 50 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b, d |
MmeI | ![]() ![]() ![]() |
TCCRAC(20/18) | CutSmart® Buffer + SAM | 50 | 100 | 50 | 100 | 65°C | 37°C | B | ![]() |
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ΦX174 RF I DNA | b, c |
MnlI | ![]() ![]() |
CCTC(7/6) | CutSmart® Buffer | 75 | 100 | 50 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | b |
MscI | ![]() ![]() |
TGG/CCA | CutSmart® Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | C | ![]() |
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λ DNA | |
MseI | ![]() ![]() |
T/TAA | CutSmart® Buffer | 75 | 100 | 75 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
MslI | ![]() ![]() |
CAYNN/NNRTG | CutSmart® Buffer | 50 | 50 | <10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
MspA1I | ![]() ![]() ![]() |
CMG/CKG | CutSmart® Buffer | 10 | 50 | 10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
MspI | ![]() ![]() |
C/CGG | CutSmart® Buffer | 75 | 100 | 50 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
MspJI | ![]() |
CNNR(9/13) | CutSmart® Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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pBR322 (dcm+) DNA | 1, e |
MwoI | ![]() ![]() ![]() |
GCNNNNN/NNGC | CutSmart® Buffer | <10 | 100 | 100 | 100 | No | 60°C | B | ![]() |
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λ DNA | |
NaeI | ![]() ![]() |
GCC/GGC | CutSmart® Buffer | 25 | 25 | <10 | 100 | No | 37°C | A | ![]() |
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pXba DNA | b |
NarI | ![]() ![]() |
GG/CGCC | CutSmart® Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | |
Nb.BbvCI | ![]() |
CCTCAGC | CutSmart® Buffer | 25 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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supercoiled plasmid DNA | e |
Nb.BsmI | ![]() |
GAATGC | NEBuffer™ 3.1 | <10 | 50 | 100 | 10 | 80°C | 65°C | A | ![]() |
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supercoiled plasmid pBR322 DNA | e |
Nb.BsrDI | ![]() |
GCAATG | CutSmart® Buffer | 25 | 100 | 100 | 100 | 80°C | 65°C | A | ![]() |
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supercoiled pUC19 DNA | e |
Nb.BssSI | ![]() |
CACGAG | NEBuffer™ 3.1 | 10 | 100 | 100 | 25 | No | 37°C | B | ![]() |
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supercoiled pUC19 DNA | e |
Nb.BtsI | ![]() |
GCAGTG | CutSmart® Buffer | 75 | 100 | 75 | 100 | 80°C | 37°C | A | ![]() |
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supercoiled pUC101 DNA (dam-/dcm-) | e |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
NciI | ![]() ![]() ![]() |
CC/SGG | CutSmart® Buffer | 100 | 25 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA | b |
NcoI § | ![]() ![]() |
C/CATGG | NEBuffer™ 3.1 | 100 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | |
NcoI-HF® | ![]() ![]() ![]() |
C/CATGG | CutSmart® Buffer | 50 | 100 | 10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
NdeI | ![]() ![]() |
CA/TATG | CutSmart® Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
NgoMIV | ![]() ![]() ![]() |
G/CCGGC | CutSmart® Buffer | 100 | 50 | 10 | 100 | No | 37°C | A | ![]() |
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pXba DNA | 1 |
NheI § | ![]() ![]() ![]() |
G/CTAGC | NEBuffer™ 2.1 | 100 | 100 | 10 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA (HindIII digest) | |
NheI-HF® | ![]() ![]() ![]() ![]() |
G/CTAGC | CutSmart® Buffer | 100 | 25 | 10 | 100 | 80°C | 37°C | C | ![]() |
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λ DNA (HindIII digest) | |
NlaIII | ![]() ![]() |
CATG/ | CutSmart® Buffer | <10 | <10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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ΦX174 RF I DNA | |
NlaIV | ![]() ![]() ![]() |
GGN/NCC | CutSmart® Buffer | 10 | 10 | 10 | 100 | 65°C | 37°C | B | ![]() |
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pBR322 DNA | |
NmeAIII | ![]() |
GCCGAG(21/19) | CutSmart® Buffer + SAM | 10 | 10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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ΦX174 RF I DNA | c |
NotI § | ![]() ![]() ![]() |
GC/GGCCGC | NEBuffer™ 3.1 | <10 | 50 | 100 | 25 | 65°C | 37°C | C | ![]() |
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pBC4 DNA | |
NotI-HF® | ![]() ![]() ![]() ![]() |
GC/GGCCGC | CutSmart® Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | ![]() |
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pBC4 DNA | |
NruI § | ![]() ![]() ![]() ![]() |
TCG/CGA | NEBuffer™ 3.1 | <10 | 10 | 100 | 10 | No | 37°C | A | ![]() |
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λ DNA | b |
NruI-HF® | ![]() ![]() ![]() ![]() ![]() |
TCG/CGA | CutSmart® Buffer | 0 | 25 | 50 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
NsiI § | ![]() ![]() |
ATGCA/T | NEBuffer™ 3.1 | 10 | 75 | 100 | 25 | 65°C | 37°C | B | ![]() |
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λ DNA | |
NsiI-HF® | ![]() ![]() ![]() |
ATGCA/T | CutSmart® Buffer | <10 | 20 | <10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
NspI | ![]() ![]() |
RCATG/Y | CutSmart® Buffer | 100 | 100 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
Nt.AlwI | ![]() ![]() |
GGATC(4/-5) | CutSmart® Buffer | 10 | 100 | 100 | 100 | 80°C | 37°C | A | ![]() |
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pUC101 DNA (dam-/dcm-) | e |
Nt.BbvCI | ![]() ![]() |
CCTCAGC(-5/-7) | CutSmart® Buffer | 50 | 100 | 10 | 100 | 80°C | 37°C | A | ![]() |
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supercoiled plasmid DNA | e |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
Nt.BsmAI | ![]() ![]() |
GTCTC(1/-5) | CutSmart® Buffer | 100 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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supercoiled plasmid DNA | e |
Nt.BspQI | ![]() |
GCTCTTC(1/-7) | NEBuffer™ 3.1 | <10 | 25 | 100 | 10 | 80°C | 50°C | B | ![]() |
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supercoiled pUC19 DNA | e |
Nt.BstNBI | ![]() |
GAGTC(4/-5) | NEBuffer™ 3.1 | 0 | 10 | 100 | 10 | 80°C | 55°C | A | ![]() |
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T7 DNA | e |
Nt.CviPII | ![]() ![]() |
(0/-1)CCD | CutSmart® Buffer | 10 | 100 | 25 | 100 | 65°C | 37°C | A | ![]() |
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pUC19 DNA | e |
PacI | ![]() ![]() |
TTAAT/TAA | CutSmart® Buffer | 100 | 75 | 10 | 100 | 65°C | 37°C | A | ![]() |
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pNEB193 DNA | |
PaeR7I | ![]() ![]() ![]() |
C/TCGAG | CutSmart® Buffer | 25 | 100 | 10 | 100 | No | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
PciI | ![]() |
A/CATGT | NEBuffer™ 3.1 | 50 | 75 | 100 | 50* | 80°C | 37°C | B | ![]() |
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pXba DNA | |
PflFI | ![]() ![]() |
GACN/NNGTC | CutSmart® Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | ![]() |
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pBC4 DNA | b |
PflMI | ![]() ![]() ![]() |
CCANNNN/NTGG | NEBuffer™ 3.1 | 0 | 100 | 100 | 50 | 65°C | 37°C | A | ![]() |
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λ DNA | 3, b, d |
PI-PspI | ![]() |
TGGCAAACAGCTATTATGGGTATTATGGGT(-13/-17) | NEBuffer™ PI-PspI + BSA | 10 | 10 | 10 | 10 | No | 65°C | B | pAKR7 XmnI-linearized Control Plasmid | ||||
PI-SceI | ![]() |
ATCTATGTCGGGTGCGGAGAAAGAGGTAAT(-15/-19) | NEBuffer™ PI-SceI + BSA | 10 | 10 | 10 | 10 | 65°C | 37°C | B | pBSvdeX XmnI-linearized Control Plasmid | ||||
PleI | ![]() ![]() |
GAGTC(4/5) | CutSmart® Buffer | 25 | 50 | 25 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b, d |
PluTI | ![]() ![]() |
GGCGC/C | CutSmart® Buffer | 100 | 25 | <10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | b |
PmeI | ![]() ![]() ![]() |
GTTT/AAAC | CutSmart® Buffer | <10 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
PmlI | ![]() ![]() ![]() |
CAC/GTG | CutSmart® Buffer | 100 | 50 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) DNA | |
PpuMI | ![]() ![]() ![]() |
RG/GWCCY | CutSmart® Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | ![]() |
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λ DNA (HindIII digest) | |
PshAI | ![]() ![]() ![]() |
GACNN/NNGTC | CutSmart® Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
PsiI-v2 | ![]() ![]() ![]() |
TTA/TAA | CutSmart® Buffer | 25 | 50 | 10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | 3 |
PspGI | ![]() ![]() |
/CCWGG | CutSmart® Buffer | 25 | 100 | 50 | 100 | No | 75°C | A | ![]() |
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T7 DNA | 3 |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
PspOMI | ![]() ![]() ![]() |
G/GGCCC | CutSmart® Buffer | 10 | 10 | <10 | 100 | 65°C | 37°C | B | ![]() |
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pXba DNA | |
PspXI | ![]() ![]() |
VC/TCGAGB | CutSmart® Buffer | <10 | 100 | 25 | 100 | No | 37°C | B | ![]() |
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λ DNA (HindIII digest) | |
PstI § | ![]() ![]() |
CTGCA/G | NEBuffer™ 3.1 | 75 | 75 | 100 | 50* | 80°C | 37°C | C | ![]() |
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λ DNA | |
PstI-HF® | ![]() ![]() ![]() |
CTGCA/G | CutSmart® Buffer | 10 | 75 | 50 | 100 | No | 37°C | C | ![]() |
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λ DNA | |
PvuI § | ![]() ![]() ![]() |
CGAT/CG | NEBuffer™ 3.1 | <10 | 25 | 100 | <10 | No | 37°C | B | ![]() |
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pXba DNA | |
PvuI-HF® | ![]() ![]() ![]() ![]() |
CGAT/CG | CutSmart® Buffer | 25 | 100 | 100 | 100 | No | 37°C | B | ![]() |
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pXba DNA | |
PvuII § | ![]() ![]() |
CAG/CTG | NEBuffer™ 3.1 | 50 | 100 | 100 | 100* | No | 37°C | B | ![]() |
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λ DNA | |
PvuII-HF® | ![]() ![]() ![]() |
CAG/CTG | CutSmart® Buffer | <10 | <10 | <10 | 100 | No | 37°C | B | ![]() |
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λ DNA | |
RsaI | ![]() ![]() ![]() |
GT/AC | CutSmart® Buffer | 25 | 50 | <10 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
RsrII | ![]() ![]() |
CG/GWCCG | CutSmart® Buffer | 25 | 75 | 10 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA | |
SacI § | ![]() ![]() |
GAGCT/C | NEBuffer™ 1.1 | 100 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
SacI-HF® | ![]() ![]() ![]() ![]() |
GAGCT/C | CutSmart® Buffer | 10 | 50 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
SacII | ![]() ![]() ![]() |
CCGC/GG | CutSmart® Buffer | 10 | 100 | 10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | |
SalI § | ![]() ![]() ![]() |
G/TCGAC | NEBuffer™ 3.1 | <10 | <10 | 100 | <10 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
SalI-HF® | ![]() ![]() ![]() ![]() |
G/TCGAC | CutSmart® Buffer | 10 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | |
SapI | ![]() ![]() |
GCTCTTC(1/4) | CutSmart® Buffer | 75 | 50 | <10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
Sau3AI | ![]() ![]() |
/GATC | NEBuffer™ 1.1 | 100 | 50 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
Sau96I | ![]() ![]() ![]() |
G/GNCC | CutSmart® Buffer | 50 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
SbfI § | ![]() ![]() |
CCTGCA/GG | CutSmart® Buffer | 50 | 25 | <10 | 100 | 80°C | 37°C | A | ![]() |
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λ DNA | 3 |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
SbfI-HF® | ![]() ![]() ![]() |
CCTGCA/GG | CutSmart® Buffer | 50 | 25 | <10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
ScaI-HF® | ![]() ![]() ![]() |
AGT/ACT | CutSmart® Buffer | 100 | 100 | 10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA | |
ScrFI | ![]() ![]() ![]() |
CC/NGG | CutSmart® Buffer | 100 | 100 | 100 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA | 2, a |
SexAI | ![]() ![]() |
A/CCWGGT | CutSmart® Buffer | 100 | 75 | 50 | 100 | 65°C | 37°C | A | ![]() |
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pBC4 DNA (dcm-) | 3, b, d |
SfaNI | ![]() ![]() |
GCATC(5/9) | NEBuffer™ 3.1 | <10 | 75 | 100 | 25 | 65°C | 37°C | B | ![]() |
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ΦX174 RF I DNA | 3, b |
SfcI | ![]() |
C/TRYAG | CutSmart® Buffer | 75 | 50 | 25 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | 3 |
SfiI | ![]() ![]() ![]() ![]() |
GGCCNNNN/NGGCC | CutSmart® Buffer | 25 | 100 | 50 | 100 | No | 50°C | C | ![]() |
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pXba DNA | |
SfoI | ![]() ![]() ![]() ![]() |
GGC/GCC | CutSmart® Buffer | 50 | 100 | 100 | 100 | No | 37°C | B | ![]() |
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λ DNA (HindIII digest) | |
SgrAI | ![]() ![]() |
CR/CCGGYG | CutSmart® Buffer | 100 | 100 | 10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | 1 |
SmaI | ![]() ![]() ![]() |
CCC/GGG | CutSmart® Buffer | <10 | <10 | <10 | 100 | 65°C | 25°C | B | ![]() |
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λ DNA (HindIII digest) | b |
SmlI | ![]() |
C/TYRAG | CutSmart® Buffer | 25 | 75 | 25 | 100 | No | 55°C | A | ![]() |
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λ DNA | b |
SnaBI | ![]() ![]() |
TAC/GTA | CutSmart® Buffer | 50* | 50 | 10 | 100 | 80°C | 37°C | A | ![]() |
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T7 DNA | 1 |
SpeI § | ![]() ![]() |
A/CTAGT | CutSmart® Buffer | 75 | 100 | 25 | 100 | 80°C | 37°C | C | ![]() |
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pXba-XbaI DNA | |
SpeI-HF® | ![]() ![]() ![]() |
A/CTAGT | CutSmart® Buffer | 25 | 50 | 10 | 100 | 80°C | 37°C | C | ![]() |
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pXba-XbaI DNA | |
SphI § | ![]() |
GCATG/C | NEBuffer™ 2.1 | 100 | 100 | 50 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | 2 |
SphI-HF® | ![]() ![]() ![]() |
GCATG/C | CutSmart® Buffer | 50 | 25 | 10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
SrfI | ![]() ![]() ![]() ![]() |
GCCC/GGGC | CutSmart® Buffer | 10 | 50 | 0 | 100 | 65°C | 37°C | B | ![]() |
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pNEB193-SrfI DNA | |
SspI § | ![]() ![]() |
AAT/ATT | NEBuffer™ SspI | 50 | 100 | 50 | 50 | 65°C | 37°C | C | ![]() |
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λ DNA | |
SspI-HF® | ![]() ![]() ![]() |
AAT/ATT | CutSmart® Buffer | 25 | 100 | <10 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
Enzyme | Sequence | Supplied NEBuffer | % Activity in NEBuffer | Heat Inac. | Incu. Temp. | Diluent | Dam | Dcm | CpG | Unit Substrate | Notes | ||||
1.1 | 2.1 | 3.1 | CutSmart | ||||||||||||
StuI | ![]() ![]() ![]() |
AGG/CCT | CutSmart® Buffer | 50 | 100 | 50 | 100 | No | 37°C | A | ![]() |
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λ DNA | |
StyD4I | ![]() ![]() ![]() ![]() |
/CCNGG | CutSmart® Buffer | 10 | 100 | 100 | 100 | 65°C | 37°C | B | ![]() |
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λ DNA | |
StyI § | ![]() ![]() |
C/CWWGG | NEBuffer™ 3.1 | 10 | 25 | 100 | 10 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
StyI-HF® | ![]() ![]() ![]() |
C/CWWGG | CutSmart® Buffer | 25 | 100 | 25 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | |
SwaI | ![]() ![]() |
ATTT/AAAT | NEBuffer™ 3.1 | 10 | 10 | 100 | 10 | 65°C | 25°C | B | ![]() |
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pXba DNA | b, d |
TaqI-v2 | ![]() ![]() ![]() |
T/CGA | CutSmart® Buffer | 50 | 100 | 50 | 100 | No | 65°C | B | ![]() |
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λ DNA | |
TfiI | ![]() ![]() ![]() |
G/AWTC | CutSmart® Buffer | 50 | 100 | 100 | 100 | No | 65°C | C | ![]() |
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λ DNA | |
TseI | ![]() ![]() |
G/CWGC | CutSmart® Buffer | 75 | 100 | 100 | 100 | No | 65°C | B | ![]() |
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λ DNA | 3 |
Tsp45I | /GTSAC | CutSmart® Buffer | 100 | 50 | <10 | 100 | No | 65°C | A | ![]() |
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λ DNA | ||
TspMI | ![]() ![]() |
C/CCGGG | CutSmart® Buffer | 50* | 75* | 50* | 100 | No | 75°C | B | ![]() |
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pUCAdeno plasmid DNA | d |
TspRI | ![]() ![]() |
NNCASTGNN/ | CutSmart® Buffer | 25 | 50 | 25 | 100 | No | 65°C | B | ![]() |
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λ DNA | |
Tth111I | ![]() ![]() |
GACN/NNGTC | CutSmart® Buffer | 25 | 100 | 25 | 100 | No | 65°C | B | ![]() |
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pBC4 DNA | b |
XbaI | ![]() ![]() ![]() |
T/CTAGA | CutSmart® Buffer | <10 | 100 | 75 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (dam-/Hind III digest) | |
XcmI | ![]() |
CCANNNNN/NNNNTGG | NEBuffer™ 2.1 | 10 | 100 | 25 | 100 | 65°C | 37°C | C | ![]() |
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λ DNA | 2 |
XhoI | ![]() ![]() ![]() |
C/TCGAG | CutSmart® Buffer | 75 | 100 | 100 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA (HindIII digest) | b |
XmaI | ![]() ![]() ![]() |
C/CCGGG | CutSmart® Buffer | 25 | 50 | <10 | 100 | 65°C | 37°C | A | ![]() |
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pXba DNA | 3 |
XmnI | ![]() ![]() |
GAANN/NNTTC | CutSmart® Buffer | 50 | 75 | <10 | 100 | 65°C | 37°C | A | ![]() |
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λ DNA | b |
ZraI | ![]() ![]() |
GAC/GTC | CutSmart® Buffer | 100 | 25 | 10 | 100 | 80°C | 37°C | B | ![]() |
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λ DNA |
§ An HF version of this enzyme is available.
Ligation and Recutting Notes
The following notes appear with any enzymes having ligation efficiencies lower than 100% as assessed by ligation and recutting.
a. Ligation is less than 10%.
b. Ligation is 25% -75%.
c. Recutting after ligation is less than 5%.
d. Recutting after ligation is 50% -75%.
e. Ligation and recutting after ligation is not applicable since the enzyme is either a nicking enzyme, is affected by methylation, or if the enzyme cleaves outside its recognition sequence.
Star Activity Notes
The following notes appear with any enzymes when star activity is a concern.
1. Star Activity may result from extended digestion, high enzyme concentration or a glycerol concentration of > 5%.
2. Star Activity may result from extended digestion.
3. Star Activity may result from a glycerol concentration of > 5%.
*. May exhibit star activity in this buffer.
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