
Time-Saver™ Qualified Restriction Enzymes
Choose Type:
- Do degenerate recognition sites need to be palindromic?
- How can I generate a restriction enzyme site map for my sequence?
- How can I search for a restriction enzyme by sequence, overhang or name?
- How should I stop my restriction digest?
- How stable is a particular restriction enzyme?
- I don't see any cleavage after my restriction digest. What factors can interfere with cleavage?
- Is extended digestion (incubation times > 1 hour) recommended?
- What does it mean to be Time-Saver™ qualified?
- What information is available in the Restriction Enzyme Database (REBASE)?
- When is star activity a concern?
- When should I choose the HF version of the enzyme?
- What are the advantages of using a RE-Mix Restriction Enzyme Master Mix?
- How should I set up a restriction digest?
- What does HF® refer to following the name of a restriction enzyme?
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
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Restriction Enzymes at NEB: Over 30 years of Innovation
- DNA Sequences and Maps Tool
- Alphabetized List of Recognition Specificities
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Cross Index of Recognition Sequences
- Dam-Dcm and CpG Methylation
- Enzymes with Multiple Recognition Sequences
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoschizomers
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Restriction Enzyme Troubleshooting Guide
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Double Digests
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Reduced Star Activities of HF® Enzymes
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction of Foreign DNA by E. coli K-12
- Site Preferences
- Star Activity
Feature Articles
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
- Enzymes powerful enough to digest in 5-15 minutes
- Flexible enough for overnight incubation
- No special formulation
- No added expense
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.