
Restriction Endonucleases
Choose Type:
- Digestion of Agarose-Embedded DNA
- Standard Digest Using RE-Mix®
- Double Digest Protocol using Two RE-Mix® Enzymes
- Optimizing Restriction Endonuclease Reactions
- Double Digest Protocol with Standard Restriction Enzymes
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
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A Modern Day Gene Genie Sir Richard Roberts on Rebase
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Restriction Endonucleases: Molecular Cloning and Beyond
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Restriction Enzyme Cleavage: ‘single-site’ enzymes and ‘multi-site’ enzymes
Restriction enzymes are proteins used to fragment and clone DNA, but their biological function is to protect bacteria and archaea against viral infections.
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Type II Restriction Enzymes: What You Need to Know | NEB
Read about Type II restriction enzymes and the distinguishing properties of the four principle subtypes.
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Whole genome assembly from next generation sequencing data using restriction and nicking enzymes in optical mapping and proximity-based ligation strategies
High throughput sequencing methods have revolutionized genomic analysis by producing millions of sequence reads from an organism’s DNA at an ever decreasing cost.
- CutSmart Trifold
- NEB Restriction Enzyme Activity Poster
- DNA Sequences and Maps Tool
- REBASE
- Alphabetized List of Recognition Specificities
- Cleavage Of Supercoiled DNA
- Compatible Cohesive Ends and Generation of New Restriction Sites
- Cross Index of Recognition Sequences
- Dam-Dcm and CpG Methylation
- Enzymes with Multiple Recognition Sequences
- Enzymes with Nonpalindromic Sequences
- Frequencies of Restriction Sites
- Interrupted Palindromes
- Isoschizomers
- Recleavable Blunt Ends
- Recleavable Filled-in 5' Overhangs
- Time-Saver™ Qualified Enzymes
- Type IIS Restriction Enzymes
- Why Choose Recombinant Enzymes?
- Restriction Enzyme Troubleshooting Guide
- Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
- Activity of Restriction Enzymes in PCR Buffers
- Alteration of Apparent Recognition Specificities Using Methylases
- Cleavage Close to the End of DNA Fragments
- Dam and Dcm Methylases of E. coli
- Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
- Double Digests
- Effects of CpG Methylation on Restriction Enzyme Cleavage
- Heat Inactivation
- NEBuffer Activity/Performance Chart with Restriction Enzymes
- Optimizing Restriction Endonuclease Reactions
- Reduced Star Activities of HF® Enzymes
- Restriction Endonucleases - Survival in a Reaction
- Restriction Enzyme Diluent Buffer Compatibility
- Restriction Enzyme Tips
- Restriction Enzymes for Droplet Digital PCR (ddPCR)
- Restriction Enzymes requiring multi-sites for efficient cleavage
- Restriction of Foreign DNA by E. coli K-12
- Site Preferences
- Star Activity
- Traditional Cloning Quick Guide
- Crystal Structure of the 8 bp-Specific Restriction Enzyme SwaI (2015)
Feature Articles
Brochures
Web Tools
Selection Tools
Troubleshooting Guides
Usage Guidelines
Posters
- Morgan, R.D., Luyten, Y.A., Johnson, S.A., Clough, E.M., Clark, T.A. and Roberts, R.J. 2016. Novel m4C modification in type I restriction-modification systems. Nucleic Acids Res. . Nov, PubMedID: 27580720, DOI:
- Fu YB, Peterson G. W., Dong Y 2016. Increasing Genome Sampling and Improving SNP Genotyping for Genotyping-by-Sequencing with New Combinations of Restriction Enzymes G3. 6:4, PubMedID: 26818077, DOI:
- Kamps-Hughes, N., Quimby, A., Zhu, Z., Johnson, E.A. 2013. Massively parallel characterization of restriction endonucleases Scopus. 41(11), PubMedID: 23605040, DOI: 10.1093/nar/gkt257
- Shah, S., Sanchez, J., Stewart, A., et al. 2015. Probing the Run-On Oligomer of Activated SgrAI Bound to DNA PLoS One. 10(4), PubMedID: 25880668, DOI: 10.1371/journal.pone.0124783.
- Loenen, W.A., Raleigh, E.A. 2014. The other face of restriction: modification-dependent enzymes. Nucleic Acids Res. 42, PubMedID: 23990325, DOI:
- Roberts, R.J., Vincze, T., Posfai, J., Macelis, D. 2014. REBASE - A database for DNA restriction and modification: enzymes, genes and genomes Nucleic Acids Research, Advance Access . , PubMedID: , DOI:
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