NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (NEB #E7103)
Even more from less, for DNA
Are you challenged with trying to obtain higher library yields using ever-decreasing input amounts? Each component in the NEBNext® Ultra™ II DNA Library Prep Kit from NEB has been reformulated, resulting in a several-fold increase in library yield with as little as 500 picograms of input DNA. These advances deliver unprecedented performance, while enabling lower inputs and fewer PCR cycles.
Get more of what you need, with the highest library yields
Use to generate high quality libraries from a broad range of input amounts, from 500 pg to 1 µg
Prepare libraries from ALL your samples, including GC-rich DNA and FFPE DNA samples
Improve yields and quality for target enrichment applications
Save time with streamlined workflows, reduced hands-on time and automation compatibility, and enjoy the flexibility of kit or module formats
The Ultra II DNA Library Prep Kit for Illumina meets the challenge of constructing high quality libraries from ever-decreasing input quantities. The reagents for each step in the library preparation workflow have been reformulated to enable high yield preparation of high quality libraries from 500 picograms to 1 microgram of input DNA. This new generation of NEBNext reagents uses a fast, streamlined, automatable workflow and enables use of fewer PCR cycles while also improving GC coverage. The kit is also compatible with PCR-free workflows and is effective with challenging samples such as FFPE DNA.
NEBNext Ultra II libraries provide the highest quality sequence data in PCR-free workflows
PCR-free libraries were generated from 100 ng of Human NA19240 genomic DNA using library prep kits shown, following manufacturers’ recommendations, and with no amplification step. Libraries were sequenced on the Illumina NextSeq 500. Reads were mapped to the GRCh37 reference using Bowtie 2.2.4. This data illustrates that NEBNext Ultra II DNA Library Prep Kit enables high quality sequence data in PCR-free workflows, even with low input amounts.
% Mapped: The percentage of reads mapped to human GRCh37 reference. % Duplication: The percentage of mapped sequence that is marked as duplicate. % Chimeras: The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.
NEBNext Ultra II enables construction of high quality libraries from FFPE DNA samples
DNA INPUT (ng)
LIBRARY YIELDS IN ng
% MAPPED IN PAIRS
Libraries were prepared from 17–30 ng of human DNA extracted from the FFPE tissue samples listed, amplified using 10 cycles of PCR and sequenced on the Illumina MiSeq.
Reads were mapped to the GRCh37 reference genome using Bowtie 2.2.4. % Mapped: The percentage of reads mapped to Human GRCh37 reference. % Mapped in Pairs: The percentage of reads whose mate pair was also aligned to the reference. % Duplication: The percentage of mapped sequence that is marked as duplicate. % Chimeras: The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.
This data illustrates that the NEBNext Ultra II DNA Library Prep Kit for Illumina enables high quality sequence data, even with low input amounts of FFPE DNA.
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
The following reagents are supplied with this product:
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.
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