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Histone H3.1/H4 Tetramer Human, Recombinant
cloned at NEB recombinant

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.

  • Highly pure, recombinant proteins
  • Mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination
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Catalog # Concentration Size
M2509S 10 µM 1 nmol
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  • Product Information

    Histone H3.1 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing. The tetramer is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.

    Human Histone H3.1 Protein Sequence: ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA CEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: AAN10051)

    Human Histone H4 Protein Sequence: SGRGK GGKGL GKGGA KRHRK VLRDN IQGIT KPAIR RLARR GGVKR ISGLI YEETR GVLKV FLENV IRDAV TYTEH AKRKT VTAMD VVYAL KRQGR TLYGF GG (Genbank accession number: AAM83108)

    SDS-PAGE analysis of Histone H3.1/H4 Tetramer Human, Recombinant
    SDS-PAGE analysis of Histone H3.1/H4 Tetramer Human, Recombinant
    Lane 1: NEB Protein Ladder (NEB #P7703 )
    Lane 2: Histone H3.1 (NEB #M2503)
    Lane 3: Histone H4 (NEB #M2504)
    Lane 4–6: 2.0, 4.0, 8.0 µg Histone H3.1/H4 Tetramer

    Product Source

    Purified H3.1 and H4 (NEB #M2503 and NEB #M2504) were denatured, refolded and the Histone H3/H4 Tetramer was purified by gel filtration.

    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    This product is related to the following categories:
    Histones Products
    This product can be used in the following applications:
    Histones & Nucleosome Assembly
    • Properties & Usage
    • Related Products
    • Product Notes
    • References
  • Citations & Technical Literature
  • Quality, Safety & Legal
    • Quality Control Assays
    • Certificate of Analysis
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