HomeNEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
Note that Sample Purification beads are not included in this kit. If beads are required, we recommend ordering the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775).
Do you prefer to use non-strand-specific library prep methods, but need increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA? To address these challenges, our next generation of non-directional RNA library prep kit has been reformulated at each step, resulting in several fold higher yields of high quality libraries and enabling use of lower input amounts and fewer PCR cycles.
The Ultra II RNA Library Prep Kit for Illumina is designed for non-directional (non-strand-specific) RNA library construction, and delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) mRNA enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1µg.
Harcourt, J., et al. (2020) Severe Acute Respiratory Coronavirus 2 from patient with Coronavirus Disease, United States Emerg Infect Dis; 26 (2), 1266-1273. DOI: https://dx.doi.org/10.3201/eid2606.200516
Manning, J., et al. (2020) ARS-CoV-2 enrichment sequencing by spiked primer MSSPE method V.4 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.beshjeb6
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