Library yield is one important measure of library preparation success, and the revision of each step in the RNA library prep workflow enables substantially higher yields with the NEBNext Ultra II RNA Library Prep Kit compared to earlier NEBNext kits, as well as another commercially available kit. Achieving sufficient yields from low input amounts can be especially challenging. However, the high efficiencies of the NEBNext Ultra II workflow overcomes these challenges, and users can now obtain higher library yields with lower input amounts for RNA prepared by poly(A) mRNA enrichment or rRNA depletion workflows. Further, samples of low quality, such as FFPE RNA, can also be used successfully, especially in conjunction with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat).
Generate the highest yields, from a wide range of input amounts
NEBNext Ultra II RNA generates yields several fold higher than the original Ultra RNA
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NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation.