This video demonstrates how the NEBuilder Assembly Tool can be used to generate overlap sequences for the assembly of two fragments into a vector. In this scenario, all fragments are amplified by PCR and the vector does not have convenient restriction sites.
Tags DNA Modifying Enzymes and Cloning Technologies
Related Products Gibson Assembly® Master Mix, NEBuilder® HiFi DNA Assembly Master Mix, NEBuilder® HiFi DNA Assembly Bundle for Large Fragments, Gibson Assembly® Cloning Kit, NEBuilder® HiFi DNA Assembly Cloning Kit