Customers often ask what protocol adjustments should I make when purifying a large plasmid?
For plasmids that are larger than 10 kb heating the elution buffer to 50 degrees Celsius prior to adding it to the column can improve yields.
Remember if you have any additional questions you can always contact our tech support team at info@neb.com
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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