Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card.

Note: These diluents are not for use with Thermophilic DNA Polymerases.

Diluent Buffer A
Diluent Buffer A					Acc65I	AccI	AciI	AflII	AgeI-HF®
AhdI	AlwI	AlwNI	ApaI	ApaLI
ApoI^§	AscI	AvaI	AvaII	BaeGI
BaeI	BamHI^§	BamHI-HF®	BanI	BanII
BccI	BceAI	BcgI	BclI^§	BglII
BlpI	BsaJI	BsaWI	BseRI	BsiEI
BsiHKAI	BslI	BsmFI	BsmI	BsoBI
Bsp1286I	BspCNI	BspDI	BspHI	BsrBI
BsrDI	BsrGI^§	BsrGI-HF®	BssKI	BstAPI
BstBI	BstEII ^§	BstEII-HF®	BstNI	BstUI
BstYI	BstZ17I-HF^®	BtgZI	BtsI	BtsIMutI
BtsI-v2	ClaI	CspCI	CviKI-1	DraI
DrdI	EaeI	EciI	Eco53kI	EcoNI
EcoO109I	EcoP15I	EcoRV^§	FatI	FauI
Fnu4HI	FokI	HaeII	HaeIII	HgaI
HhaI	HinfI	HinP1I	HpaI	HpaII
Hpy188I	Hpy99I	HpyCH4III	HpyCH4IV	HpyCH4V
KpnI^§	KpnI-HF®	MboI	MfeI^§	MfeI-HF®
MluCI	MluI^§	MluI-HF®	MlyI	MseI
MslI	MspI	NaeI	NarI	Nb.BbvCI
Nb.BsmI	Nb.BsrDI	Nb.BtsI	NciI	NcoI^§
NdeI	NgoMIV	NotI-HF®	NruI^§	NruI-HF®
NspI	Nt.AlwI	Nt.BbvCI	Nt.BsmAI	Nt.BstNBI
Nt.CviPII	PacI	PaeR7I	PflFI	PflMI
PhoI	PleI	PluTI	PmeI	PmlI
PshAI	PspGI	RsaI	SacI^§	SacI-HF®
SacII	SalI^§	SalI-HF®	Sau3AI	Sau96I
SbfI^§	ScaI^§	SexAI	SgrAI	SmlI
SnaBI	StuI	StyI^§	StyI-HF®	Tsp45I
Tsp509I	XbaI	XhoI	XmaI	XmnI

Diluent Buffer B
Diluent Buffer B					AatII	AclI	AcuI	AfeI	AflIII
AleI	AleI-v2	AluI	ApeKI	ApoI-HF
AseI	AsiSI	AvrII	BbsI^§	BbsI-HF^®
BbvCI	BbvI	BclI-HF	BcoDI	BfaI
BfuAI	BfuCI	BglI	BmgBI	BmrI
BmtI^§	BmtI-HF®	BpmI	Bpu10I	BpuEI
BsaBI	BsaI^§	BsaI-HF®	BsaI-HF®v2	BseYI
BsgI	BsiWI^§	BsiWI-HF^®	BsmAI	BsmBI
BsmBI-v2	BspEI	BspMI	BspQI	BsrI
BssHII	BssSI	BssSI-v2	BstXI	BstZ17I^§
BtgI	BtsCI	Cac8I	DdeI	DpnI
DpnII	DraIII^§	DraIII-HF®	EagI^§	EagI-HF®
EarI	EcoRV-HF®	Esp3I	FseI	FspEI
HincII	HindIII^§	HindIII-HF®	HphI	Hpy188III
I-CeuI	I-SceI	KasI	LpnPI	MmeI
MnlI	MspA1I	MspJI	MwoI	Nb.BssSI
NcoI-HF®	NlaIII	NlaIV	NmeAIII	NsiI^§
NsiI-HF®	Nt.BspQI	PciI	PI-PspI	PI-SceI
PpuMI	PsiI	PsiI-v2	PspOMI	PspXI
PvuI^§	PvuI-HF®	PvuII^§	PvuII-HF®	SapI
SbfI-HF®	ScaI-HF®	SfaNI	SfcI	SfoI
SmaI	SphI^§	SphI-HF®	SrfI	SspI-HF®
StyD4I	SwaI	TaqI-v2	TliI	TseI
TspMI	TspRI	Tth111I	ZraI

Diluent Buffer C
Diluent Buffer C					AbaSI	AgeI^§	BciVI	BsaAI	BsaHI
BsaXI	BsrFI	BsrFI-v2	Bsu36I	CviAII
CviQI	EcoRI^§	EcoRI-HF®	FspI	Hpy166II
MboII	MscI	NheI^§	NheI-HF®	NotI^§
PstI^§	PstI-HF®	RsrII	ScrFI	SfiI
SpeI^§	SpeI-HF®	SspI^§	TfiI	XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.

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