Restriction Enzyme Diluent Buffer Compatibility

Diluent Buffers (A, B or C) are recommended for making dilutions of restriction endonucleases. When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card. 

Note: These diluents are not for use with Thermophilic DNA Polymerases.

Diluent Buffer A
Acc65I AccI AciI AflII AgeI-HF®
AhdI AlwI AlwNI ApaI ApaLI
ApoI§ AscI AvaI AvaII BaeGI
BaeI BamHI§ BamHI-HF® BanI BanII
BccI BceAI BcgI BclI§ BglII
BlpI BsaJI BsaWI BseRI BsiEI
BsiHKAI BslI BsmFI BsmI BsoBI
Bsp1286I BspCNI BspDI BspHI BsrBI
BsrDI BsrGI§ BsrGI-HF® BssKI BstAPI
BstBI BstEII § BstEII-HF® BstNI BstUI
BstYI BstZ17I-HF® BtgZI BtsαI BtsI
BtsIMutI ClaI CspCI CviKI-1 DraI
DrdI EaeI EciI Eco53kI EcoNI
EcoO109I EcoP15I EcoRV§ FatI FauI
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinfI HinP1I HpaI HpaII
Hpy188I Hpy99I HpyCH4III HpyCH4IV HpyCH4V
KpnI§ KpnI-HF® MboI MfeI§ MfeI-HF®
MluCI MluI§ MluI-HF® MlyI MseI
MslI MspI NaeI NarI Nb.BbvCI
Nb.BsmI Nb.BsrDI Nb.BtsI NciI NcoI§
NdeI NgoMIV NotI-HF® NruI§ NruI-HF®
NspI Nt.AlwI Nt.BbvCI Nt.BsmAI Nt.BstNBI
Nt.CviPII PacI PaeR7I PflFI PflMI
PhoI PleI PluTI PmeI PmlI
PshAI PspGI RsaI SacI§ SacI-HF®
SacII SalI§ SalI-HF® Sau3AI Sau96I
SbfI§ ScaI§ SexAI SgrAI SmlI
SnaBI StuI StyI§ StyI-HF® Tsp45I
Tsp509I XbaI XhoI XmaI XmnI
Diluent Buffer B
AatII AclI AcuI AfeI AflIII
AleI R0685 AleI-v2 AluI ApeKI ApoI-HF
AseI AsiSI AvrII BbsI§ BbsI-HF®
BbvCI BbvI BclI-HF BcoDI BfaI
BfuAI BfuCI BglI BmgBI BmrI
BmtI§ BmtI-HF® BpmI Bpu10I BpuEI
BsaBI BsaI§ BsaI-HF® BsaI-HF®v2 BseYI
BsgI BsiWI§ BsiWI-HF® BsmAI BsmBI
BspEI BspMI BspQI BsrI BssHII
BssSαI BssSI BstXI BstZ17I§ BtgI
BtsCI Cac8I DdeI DpnI DpnII
DraIII§ DraIII-HF® EagI§ EagI-HF® EarI
EcoRV-HF® Esp3I FseI FspEI HincII
HindIII§ HindIII-HF® HphI Hpy188III I-CeuI
I-SceI KasI LpnPI MmeI MnlI
MspA1I MspJI MwoI Nb.BssSI NcoI-HF®
NlaIII NlaIV NmeAIII NsiI§ NsiI-HF®
Nt.BspQI PciI PI-PspI PI-SceI PpuMI
PsiI PspOMI PspXI PvuI§ PvuI-HF®
PvuII§ PvuII-HF® SapI SbfI-HF® ScaI-HF®
SfaNI SfcI SfoI SmaI SphI§
SphI-HF® SrfI SspI-HF® StyD4I SwaI
TaqαI TliI TseI TspMI TspRI
Tth111I ZraI
Diluent Buffer C
AbaSI AgeI§ BciVI BsaAI BsaHI
BsaXI BsrFαI BsrFI Bsu36I CviAII
CviQI EcoRI§ EcoRI-HF® FspI Hpy166II
MboII MscI NheI§ NheI-HF® NotI§
PstI§ PstI-HF® RsrII ScrFI SfiI
SpeI§ SpeI-HF® SspI§ TfiI XcmI

§ A HF version of this enzyme is available.
HF® is a registered trademark of New England Biolabs, Inc.

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