The following table indicates the number of units of HF®-enzyme that can be used compared to the wild type counterpart before any significant star activity is detected. The HF Factor refers to the X-fold increase that is achieved by choosing an HF enzyme. This data clearly illustrates the flexibility that is offered by using an HF restriction enzyme.
§ A HF version of this enzyme is available.
† Wild type enzymes were tested in supplied buffer for comparisons.
* Wei, H. et al. (2008) Nucleic Acids Res. 36, e50.
HF® is a registered trademark of New England Biolabs, Inc.