Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

Please also check these additional information about:
RE Performance Chart | Double Digestion | Time-Saver Enzymes

Enzyme Optimal Temperature % Activity at 37 °C
AbaSI 25°C 0
ApaI 25°C 100
ApeKI 75°C 10
ApoI§ 50°C 50
BaeI 25°C 100
BclI§ 50°C 50
BfuAI 50°C 25
BsaBI 60°C 25
BsaJI 60°C 25
BsaWI 60°C 50
BsiEI 60°C 10
BsiHKAI 65°C 10
BsiWI§ 55°C 25
BslI 55°C 50
BsmAI 55°C 50
BsmBI-v2 55°C 10
BsmFI 65°C 100
BsmI 65°C 10
BspQI 50°C 50
BsrDI 65°C 50
BsrI 65°C 10
BssHII 50°C 100
BstAPI 60°C 25
BstBI 65°C 10
BstEII § 60°C 10
BstNI 60°C 25
BstUI 60°C 10
BstYI 60°C 10
BtgZI 60°C 50
BtsCI 50°C 25
BtsIMutI 55°C 50
BtsI-v2 55°C 100
CviAII 25°C 10
CviQI 25°C 25
FatI 55°C 100
FauI 55°C 50
MwoI 60°C 25
Nb.BsmI 65°C 100
Nb.BsrDI 65°C 50
Nt.BspQI 50°C 50
Nt.BstNBI 55°C 50
PI-PspI 65°C 10
PspGI 75°C 25
SfiI 50°C 10
SmaI 25°C 25
SmlI 55°C 10
SwaI 25°C 25
TaqI-v2 65°C 10
TfiI 65°C 10
TseI 65°C 10
Tsp45I 65°C 10
TspMI 75°C 10
TspRI 65°C 10
Tth111I 65°C 10

§ An HF version of this enzyme is available.