Recommended HiScribe RNA Synthesis Kits by Application
T7 KITS | SP6 KITS | ||||||
---|---|---|---|---|---|---|---|
APPLICATION | HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040) | HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050) | HISCRIBE T7 ARCA mRNA KIT (#E2065) | HISCRIBE T7 ARCA mRNA KIT (WITH TAILING) (#E2060) | HISCRIBE T7 mRNA KIT WITH CLEANCAP® REAGENT AG (#E2080) | HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070) | |
PROBE LABELING |
Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc.
|
✓ | ✓ | ||||
Non-fluorescent labeling: Biotin, Digoxigenin
|
✓ | ✓ | |||||
High specific activity radiolabeling
• Blot hybridization • RNase protection |
✓ | ✓ | |||||
Streamlined high yield CleanCap Reagent AG capped RNA synthesis • Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation |
✓ | ||||||
mRNA & RNA FOR TRANSFECTION | Streamlined mRNA synthesis with ARCA co-transcriptional
capping and enzymatic poly(A) tailing
• Transfection • Microinjection • In vitro translation |
✓ | |||||
Streamlined ARCA capped RNA synthesis
• Template encoded poly(A) tails • Non polyadenylated transcripts • Transfection • Microinjection • In vitro translation |
✓ | ||||||
Co-transcriptional capping with alternate cap analogs
• Transfection • Microinjection • In vitro translation |
✓ | ✓ | |||||
Post-transcriptional capping with Vaccinia mRNA Capping System
• Transfection • Microinjection • In vitro translation |
✓ | ✓ | ✓ | ||||
Complete substitution of NTPs: 5-mC, pseudouridine, etc.
• Post-transcriptional capping with Vaccinia mRNA Capping System |
✓ | ✓ | |||||
Partial substitution of NTPs: 5-mC, pseudouridine, etc. | ✓ | ✓ | ✓ | ✓ | |||
Unmodified RNA | ✓ | ✓ | |||||
Hairpins, short RNA, dsRNA
• Gene knockdown |
✓ | ✓ | |||||
STRUCTURE, FUNCTION, & BINDING STUDIES | Complete substitution of NTPs
• Aptamer selection
• Isotopic labeling |
✓ | ✓ | ||||
Partial substitution of one or more NTPs
• Aptamer selection • Structure determination |
✓ | ||||||
Unmodified RNA
• SELEX • Structure determination |
✓ | ✓ |