Recommended HiScribe RNA Synthesis Kits by Application

  T7 KITS SP6 KITS
APPLICATION HISCRIBE T7 HIGH YIELD RNA SYNTHESIS KIT (#E2040) HISCRIBE T7 QUICK HIGH YIELD RNA SYNTHESIS KIT (#E2050) HISCRIBE T7 ARCA mRNA KIT (#E2065) HISCRIBE T7 ARCA mRNA KIT (WITH TAILING) (#E2060) HISCRIBE T7 mRNA KIT WITH CLEANCAP®  REAGENT AG  (#E2080) HISCRIBE SP6 RNA SYNTHESIS KIT (#E2070)
PROBE LABELING

Fluorescent labeling: FAM, Cyanine (Cy) dyes, etc.
• Fluorescent in situ hybridization (FISH)

       

Non-fluorescent labeling: Biotin, Digoxigenin
In situ hybridization
• Blot hybridization with secondary detection
• Microarray

       
High specific activity radiolabeling
• Blot hybridization
• RNase protection
       
  Streamlined high yield CleanCap Reagent AG capped RNA synthesis
• Template encoded poly(A) tails
• Non polyadenylated transcripts
• Transfection
• Microinjection
In vitro translation
       
mRNA & RNA FOR TRANSFECTION Streamlined mRNA synthesis with ARCA co-transcriptional capping and enzymatic poly(A) tailing
• Transfection
• Microinjection
In vitro translation
       
Streamlined ARCA capped RNA synthesis
• Template encoded poly(A) tails
• Non polyadenylated transcripts
• Transfection
• Microinjection
In vitro translation
       
Co-transcriptional capping with alternate cap analogs
• Transfection
• Microinjection
In vitro translation
       
Post-transcriptional capping with Vaccinia mRNA Capping System
• Transfection
• Microinjection
In vitro translation
     
Complete substitution of NTPs: 5-mC, pseudouridine, etc.
• Post-transcriptional capping with Vaccinia mRNA Capping System
       
Partial substitution of NTPs: 5-mC, pseudouridine, etc.    
Unmodified RNA        
Hairpins, short RNA, dsRNA
• Gene knockdown
       
STRUCTURE, FUNCTION, & BINDING STUDIES Complete substitution of NTPs • Aptamer selection
• Isotopic labeling
       
Partial substitution of one or more NTPs
• Aptamer selection
• Structure determination
         
Unmodified RNA
• SELEX
• Structure determination