NEBNext® Multiplex Oligos Selection Chart

 


SINGLE INDEX


DUAL INDEX


UNIQUE DUAL INDEX

NEB Products NEB #E7335
NEB #E7500
NEB #E7710
NEB #E7730
NEB #E6609

NEB #E7600
NEB #E7780

NEB #E6440
NEB #E6442
NEB #E6444
NEB #E6446
NEB #E7140

Contains UMI

No

No

No

Addresses index hopping

No

No

Yes

Indexing Strategy

Index Primer

Index Primer

Index Primer

Applications

DNA-seq, RNA-seq 
(except Small RNA)

DNA-seq, RNA-seq
(except Small RNA)

DNA-seq, RNA-seq
(except Small RNA)

Number of indices  for multiplexing

up to 144

up to 384

up to 384

Compatible with EM-seq™

Yes*

Yes*

Yes*

Compatible with EpiMark® bisulfite sequencing

Yes**

Yes**

Yes**

Number of sets available; formats and indices available

Five;
Sets 1-4 (12 indices/set): Individual vials
96 Index: Premixed plate

Two;
individual vials containing 8 i5 primers and 12 i7 primers for combinatorial mixing

Four;
96 indices in premixed, foil-sealed 96-well plates; Including a version for EM-seq (up to 120 indices, either 96-well plate or 24 vial format).

* Requires the use of the EM-seq Adaptor; Single, dual and unique dual index are all compatible; NEB recommends using the Unique Dual Index Primers found in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) or the NEBNext Multiplex Oligos for EM-seq (NEB #E7140), both supplied with the NEBNext EM-seq Adaptor; For higher levels of multiplexing, Unique Dual Index Primers Sets 3 and 4  (NEB #E6444 and #E6446) are also validated for EM-seq.

**  Requires use of NEBNext EM-seq adaptor from NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs, #E7140S/L or NEBNext methylated adaptor from NEBNext Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1, #E7535S/L) .