The following table indicates relative activity of nicking enzymes at different temperatures. Recommended incubation is noted (and normalized at 3-stars) and relative activity to that is indicated by the number of stars. When possible, NEB has chosen 37°C as the recommended temperature for its convenience, even if increased activity could be seen at an alternative temperature. For more information on nicking enzymes, please visit Nicking Endonucleases from NEB.
|Temperature and Activity|
|-||<10% of the activity at recommended temperature|
|★||10%-50% of the activity at recommended temperature|
|★★||50%-99% of the activity at recommended temperature|
|★★★||100% of the activity at recommended temperature|
|★★★★||>100%-200% of the activity at recommended temperature|
|★★★★★||>200% of the activity at recommended temperature|
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