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Cloning Competent Cell Transformation Selection Chart

NEB's competent cells include several popular strains for cloning. Our cloning strains include derivatives of the industry standards, DH5⍺™ and DH10β™. NEB Turbo is unique to NEB and allows colony growth after 6.5 hours. NEB's dam-/dcm-strain enables Dam and Dcm methylation-free plasmid growth. NEB Stable is recommended in all difficult cloning experiments. Our cells are all extensively tested for phage resistance, antibiotic resistance and sensitivity, blue/white screening and transformation efficiency. High efficiency, 5 minute transformation and electroportation protocols are provided, when applicable.
Features Versatile ✓         ✓
Fast growth
(< 8 hours)		   ✓        
Toxic gene cloning   ✓ ✓

✓
Large plasmid/BAC cloning       ✓    
Dam/Dcm free plasmid growth         ✓  
Retroviral/lentiviral
vector cloning		           ✓
recA- ✓   ✓ ✓   ✓
endA- ✓ ✓ ✓ ✓ ✓ ✓
 Formats Chemically Competent ✓ ✓ ✓ ✓ ✓ ✓
Electrocompetent ✓ ✓   ✓    
Subcloning ✓          
96-well plate ✓          
384-well plate ✓  
     
8-tube strips ✓