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Home Tools & Resources Selection Charts Buffer and Diluent Formulation Table

Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs at 1X concentration.
Components NEBuffer™ 1.1 | B7201 NEBuffer™ 2.1 | B7202 NEBuffer™ 3.1 | B7203 CutSmart® Buffer | B7204 T4 DNA Ligase Reaction Buffer | B0202 StickTogether™ DNA Ligase Buffer | B0535 ThermoPol® Reaction Buffer Pack | B9004 T4 Polynucleotide Kinase Reaction Buffer | B0201 Antarctic Phosphatase Reaction Buffer | B0289 Exonuclease I Reaction Buffer | B0293 Exonuclease VII Reaction Buffer | B0379 Diluent A | B8001 Diluent B | B8002 Diluent C | B8003
Tris-HCl 10 mM 50 mM 50 mM 66 mM 20 mM 70 mM 50 mM 10 mM 10 mM 10 mM
Bis-Tris-Propane-HCl 10 mM 50 mM
Tris-acetate 20 mM
Sodium Phosphate
Potassium Acetate 50 mM
Magnesium Acetate 10 mM
EDTA 8 mM 0.1 mM 0.1 mM 0.1 mM
NaCl 50 mM 100 mM 300 mM 250 mM
KCl 10 mM 50 mM
MgCl2 10 mM 10 mM 10 mM 10 mM 10 mM 10 mM 1 mM 6.7 mM
ZnCl2 0.1 mM
MgSO4 2 mM
(NH4)2SO4 10 mM
Glycine-KOH 67 mM
BSA 100 µg/ml 100 µg/ml 100 µg/ml 100 µg/ml 200 µg/ml 500 µg/ml 200 µg/ml
ATP 1 mM 1 mM
DTT 10 mM 1 mM 5 mM 1 mM 1 mM 1 mM
β-ME 10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000) 7.5%
Triton® X-100 0.1% 0.15%
Glycerol 50% 50% 50%
pH @ 25 7.00 7.90 7.90 7.90 7.50 7.60 8.80 7.60 6.00 9.50 8.00 7.40 7.40 7.40

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