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Buffer and Diluent Formulation Table

The following chart lists the components of various common buffers and diluents from New England Biolabs at 1X concentration.
Components NEBuffer™ 1.1 | B7201 NEBuffer™ 2.1 | B7202 NEBuffer™ 3.1 | B7203 CutSmart® Buffer | B7204 T4 DNA Ligase Reaction Buffer | B0202 NEBNext® Quick Ligation Reaction Buffer | B6058 ThermoPol® Reaction Buffer Pack | B9004 T4 Polynucleotide Kinase Reaction Buffer | B0201 Antarctic Phosphatase Reaction Buffer | B0289 Exonuclease I Reaction Buffer | B0293 Exonuclease VII Reaction Buffer | B0379 Diluent A | B8001 Diluent B | B8002 Diluent C | B8003
Tris-HCl 10 mM 50 mM 50 mM 66 mM 20 mM 70 mM 50 mM 10 mM 10 mM 10 mM
Bis-Tris-Propane-HCl 10 mM 50 mM
Tris-acetate 20 mM
Sodium Phosphate
Potassium Acetate 50 mM
Magnesium Acetate 10 mM
EDTA 8 mM 0.1 mM 0.1 mM 0.1 mM
NaCl 50 mM 100 mM 300 mM 250 mM
KCl 10 mM 50 mM
MgCl2 10 mM 10 mM 10 mM 10 mM 10 mM 10 mM 1 mM 6.7 mM
ZnCl2 0.1 mM
MgSO4 2 mM
(NH4)2SO4 10 mM
Glycine-KOH 67 mM
BSA 100 µg/ml 100 µg/ml 100 µg/ml 100 µg/ml 200 µg/ml 500 µg/ml 200 µg/ml
ATP 1 mM 1 mM
DTT 10 mM 1 mM 5 mM 1 mM 1 mM 1 mM
β-ME 10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000) 6%
Triton® X-100 0.1% 0.15%
Glycerol 50% 50% 50%
pH @ 25 7.0 7.9 7.9 7.9 7.5 7.6 8.8 7.6 6.0 9.5 8.0 7.4 7.4 7.4

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