NEB Diluent and Buffer Table

Composition List for NEB Diluents and Buffers

You can find the composition of NEB buffers and diluents in the table below. The columns represent the diluent or buffer referenced, and the rows represent the individual diluent or buffer components. The squares within the table indicate the final concentration of components for a 1X concentration of the buffer or diluent defined by the column. The final row of the table indicates the final pH of the mixture.

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, NEB will be switching our current BSA-containing reaction buffers (NEBuffer™ 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin (rAlbumin)-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We anticipate that this switch may take as long as 6 months to complete. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. Find more details at www.neb.com/BSA-free.

 During this transition period, you may receive product with BSA or rAlbumin-containing buffers. NEB has rigorously tested both and has not seen any difference in enzyme performance when using either buffer. Either buffer can be used with your enzyme. All website content will be switched in April to reflect the changes, although you may not receive the new buffer with your product immediately.

Components NEBuffer™ EcoRI/SspI | B0101 T4 Polynucleotide Kinase Reaction Buffer | B0201 T4 DNA Ligase Reaction Buffer | B0202 Antarctic Phosphatase Reaction Buffer | B0289 Exonuclease I Reaction Buffer | B0293 Exonuclease VII Reaction Buffer | B0379 StickTogether™ DNA Ligase Buffer | B0535 NEBuffer™ r1.1 | B6001 NEBuffer™ r2.1 | B6002 NEBuffer™ r3.1 | B6003 rCutSmart™ Buffer | B6004 Diluent A | B8001 Diluent B | B8002 Diluent C | B8003 ThermoPol® Reaction Buffer Pack | B9004
Tris-HCl 100 mM 70 mM 50 mM 50 mM 66 mM 10 mM 50 mM 10 mM 10 mM 10 mM 20 mM
Bis-Tris-Propane-HCl 50 mM 10 mM
Tris-acetate 20 mM
Sodium Phosphate 50 mM
Potassium Acetate 50 mM
Magnesium Acetate 10 mM
EDTA 8 mM 0.1 mM 0.1 mM 0.1 mM
NaCl 50 mM 50 mM 100 mM 300 mM 250 mM
KCl 50 mM 10 mM
MgCl2 10 mM 10 mM 10 mM 1 mM 6.7 mM 10 mM 10 mM 10 mM 10 mM
ZnCl2 0.1 mM
MgSO4 2 mM
(NH4)2SO4 10 mM
Glycine-KOH 67 mM
BSA 200 µg/ml 500 µg/ml 200 µg/ml
Recombinant Albumin 100 µg/ml 100 µg/ml 100 µg/ml 100 µg/ml
ATP 1 mM 1 mM
DTT 5 mM 10 mM 1 mM 1 mM 1 mM 1 mM
β-ME 10 mM
2-mercapto-ethanol
Polyethylene glycol (PEG 6000) 7.5%
Triton® X-100 0.025% 0.15% 0.1%
Glycerol 50% 50% 50%
pH @ 25 7.50 7.60 7.50 6.00 9.50 8.00 7.60 7.00 7.90 7.90 7.90 7.40 7.40 7.40 8.80
Loading Spinner