Buffer and Diluent Formulation Table
| Components | T4 Polynucleotide Kinase Reaction Buffer | B0201 | T4 DNA Ligase Reaction Buffer | B0202 | Antarctic Phosphatase Reaction Buffer | B0289 | Exonuclease I Reaction Buffer | B0293 | Exonuclease VII Reaction Buffer | B0379 | StickTogether™ DNA Ligase Buffer | B0535 | NEBuffer™ 1.1 | B7201 | NEBuffer™ 2.1 | B7202 | NEBuffer™ 3.1 | B7203 | CutSmart® Buffer | B7204 | Diluent A | B8001 | Diluent B | B8002 | Diluent C | B8003 | ThermoPol® Reaction Buffer Pack | B9004 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tris-HCl | 70 mM | 50 mM | 50 mM | 66 mM | 10 mM | 50 mM | 10 mM | 10 mM | 10 mM | 20 mM | ||||
| Bis-Tris-Propane-HCl | 50 mM | 10 mM | ||||||||||||
| Tris-acetate | 20 mM | |||||||||||||
| Sodium Phosphate | ||||||||||||||
| Potassium Acetate | 50 mM | |||||||||||||
| Magnesium Acetate | 10 mM | |||||||||||||
| EDTA | 8 mM | 0.1 mM | 0.1 mM | 0.1 mM | ||||||||||
| NaCl | 50 mM | 100 mM | 300 mM | 250 mM | ||||||||||
| KCl | 50 mM | 10 mM | ||||||||||||
| MgCl2 | 10 mM | 10 mM | 1 mM | 6.7 mM | 10 mM | 10 mM | 10 mM | 10 mM | ||||||
| ZnCl2 | 0.1 mM | |||||||||||||
| MgSO4 | 2 mM | |||||||||||||
| (NH4)2SO4 | 10 mM | |||||||||||||
| Glycine-KOH | 67 mM | |||||||||||||
| BSA | 100 µg/ml | 100 µg/ml | 100 µg/ml | 100 µg/ml | 200 µg/ml | 500 µg/ml | 200 µg/ml | |||||||
| ATP | 1 mM | 1 mM | ||||||||||||
| DTT | 5 mM | 10 mM | 1 mM | 1 mM | 1 mM | 1 mM | ||||||||
| β-ME | 10 mM | |||||||||||||
| 2-mercapto-ethanol | ||||||||||||||
| Polyethylene glycol (PEG 6000) | 7.5% | |||||||||||||
| Triton® X-100 | 0.15% | 0.1% | ||||||||||||
| Glycerol | 50% | 50% | 50% | |||||||||||
| pH @ 25 | 7.60 | 7.50 | 6.00 | 9.50 | 8.00 | 7.60 | 7.00 | 7.90 | 7.90 | 7.90 | 7.40 | 7.40 | 7.40 | 8.80 |
