We are using PacBio sequencing to profile the substrate specificity of DNA ligases on end-joining substrates. Our single molecule sequence method allows us to investigate the ability of DNA ligase to discriminate against mismatches (fidelity) and sequence preferences of the enzyme (bias). We are extending this analysis to profile a variety of DNA ligases and end types, as well as to develop a deeper understanding of kinetics and thermodynamics of ligation.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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