Cleavage of ssRNA with RNase If (NEB #M0243)

1. Set-up the reaction as follows:

COMPONENTS 20 μl REACTION
 Single stranded RNA
 0.1 to 0.5 μgs
 NEBuffer 3 (10X)
 2 μl (1X)
 Nuclease-free H2O (NEB #B1500)
 up to 20 μl
 RNase If
 1 μl (50 units)

 

2. Mix thoroughly.

3. Incubate at 37°C for 15 minutes.

4. Heat Inactivation 70°C for 20 minutes.

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RNases
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RNase If