Protocol for cleavage with McrBC (M0272)

Reaction Conditions:


1X NEBuffer 2, Supplement with 200 µg/ml Recombinant Albumin and 1 mM GTP. Incubate at 37°C.

Standard Protocol:

 

  1. Set up the following reaction on ice.

    COMPONENT  50 µl REACTION 
    NEBuffer 2 (10X) 
    5 µl (1X) 
    Recombinant Albumin (20 mg/ml)
    0.5 µl  (200 µg/ml)
    GTP (100 mM)
    0.5 µl (1 mM)
    Methylated DNA
    1 µg
    McrBC 
    1 µl  (10 units)
     dH20  up to 50 μl
     

  2. Incubate at 37°C for 1-2 hours.

  3. Inactivate at 65°C for 20 minutes.

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Related Products:
McrBC