Protocol for #M0660
- Set up the following reaction.
Water up to 50 µL 10X Nuclease P1 Reaction Buffer
5 µL ssDNA or RNA Substrate
up to 1 µg
Nuclease P1 10 units
- Incubate at 37°C for 30 min.
- Inactivate- 75°C for 10 min or by the addition of 2 µL of 0.5 M EDTA.