Protocol for #M0660

  1. Set up the following reaction. 

    Water  up to 50 µL 
    10X Nuclease P1 Reaction Buffer
    5 µL 
    ssDNA or RNA Substrate
    up to 1 µg
    Nuclease P1 10 units
    (0.1 µL)


  2. Incubate at 37°C for 30 min.

  3. Inactivate- 75°C for 10 min or by the addition of 2 µL of 0.5 M EDTA.