Suggested Loading Protocol for DNA Ladders & Markers
Dilute only 1 µl of DNA Ladder at a time
- Prepare loading mixture as follows:
- Mix gently by pipetting
- Load onto the agarose gel
|Distilled water (dH20)* or TE Buffer||4 μl|
|Gel Loading Dye, Purple (6X), no SDS||1 μl|
|DNA Ladder/Marker||1 μl|
|Total volume||6 μl|
*For multiple loads, dilution, and storage, use TE or other buffer of minimal ionic strength instead of water. DNA may denature if diluted and stored in dH20.
This protocol is recommended for a 5mm wide gel lane. The components of the mixture should be scaled up or down, depending on the width of the lane.