Thermolabile USER® II Enzyme Standard Protocol (M5508)

Thermolabile USER® II Enzyme
1 unit (1 µl)
 Uracil-containing DNA
 up to 10 pmol
 CutSmart Buffer (10X)
 5 µl (1X)
 Total Reaction Volume
 50 µl
 Incubation Temperature
 37°C
 Incubation Time
 15 min.
 Heat Inactivate for 10 min at 65°C