Enzymatic PCR Cleanup Protocol
- Add 0.5 μl of Exo I and 1 μl of rSAP to 5 μl of PCR product. (Note that the Quick Dephosphorylation Kit (NEB #M0508), which contains Quick CIP, can be used in the same volume.)
- Incubate the mix at the 37°C for 15 minutes.
- Inactivate both enzymes at 80°C for 15 minutes.
- PCR products are ready for downstream application.