Enzymatic PCR Cleanup Protocol

  1.  Add 0.5 μl of Exo I and 1 μl of rSAP to 5 μl of PCR product.
  2. (Note that the Quick Dephosphorylation Kit (NEB #M0508), which contains Quick CIP, can be used in the same volume.)
  3. Incubate the mix at the 37°C for 15 minutes.
  4. Inactivate both enzymes at 80°C for 15 minutes.
  5. PCR products are ready for downstream application.

Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050).