Protocol for 9°N DNA Ligase (M0238)
|DNA||up to 1 µg
|9°N DNA Ligase Reaction Buffer (10X)
|9°N DNA Ligase||2 µl (80 units)|
|H2O||up to 50 µl|
2. Incubate at 45°C for 15 minutes.
This tutorial describes the use of the NEBioCalculator web tool to optimize the molar ratio between vector and insert DNA for use in a ligation reaction.