Validate the Library - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (For use with NEB #E7300, #E7330, and #E7580)

For use with NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1), NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2), and NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)

  1. Load 1 μl of the size selected purified library on a 2100 Bioanalyzer using a DNA 1000 or High Sensitivity DNA chip according to the manufacturer's instructions (Figure 5).

  2. Check the size, purity, and concentration of the sample.

    Figure 5: Electropherogram trace of the gel size selected purified library from human brain total RNA.

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