Denaturing PAGE/Urea or Denaturing Agarose Gel (B0363)
- Add sample to an equal volume of RNA Loading Dye, (2X). Mix well.
- Heat at 65–70°C for 5–10 minutes to denature RNA.
- While heating the samples, setup the gel box and flush urea out of the wells with running buffer using a large tip.
- Load samples.