NEBNext End Repair Module Protocol (E6050)
Starting Material: 1-5 μg of DNA Fragmented to 100-1000 bp in ≤ 85 μl
- Mix the following components in a sterile microfuge tube:
Fragmented DNA variable NEBNext End Repair Reaction Buffer (10X) 10 μl NEBNext End Repair Enzyme Mix 5 μl Sterile H2O for a final volume of 100 μl variable Total volume 100 μl
- Incubate in a thermocycler for 30 minutes at 20°C with heated lid set to
30°C (or off).
- Purify DNA Sample using AMPure XP or SPRIselect beads.