The Monarch Genomic DNA Purification Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic
DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria
and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Protocols are also included to enable
purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA.
Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The
purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has
a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms.
Cultured mammalian cells: up to 5 x 106 cells
Mammalian whole blood: 100 µl
Tissue: up to 25 mg, depending on tissue type
Bacteria: up to 2 x 109
Yeast: up to 5 x 107
Saliva: up to 500 µl
Genomic DNA requiring cleanup
30 µg genomic DNA
Varies depending on sample type
Genomic DNA Size:
Peak size > 50 kb for most sample types; may be lower for saliva and buccal swabs
< 1% (with included RNase A treatment)
≥35 µl, but 100 µl is recommended
A260/280 ≥ 1.8
A260/230 ≥ 2.0
Validated Sample Types:
Guinea Pig Blood
This product is related to the following categories:
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