Monarch^® RNA Priming Buffer

Companion Products

• Luna^® Universal One-Step RT-qPCR Kit
• Luna^® Universal Probe One-Step RT-qPCR Kit
• NEBNext^® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads
• ProtoScript® II First Strand cDNA Synthesis Kit
• NEBNext® Poly(A) mRNA Magnetic Isolation Module
• NEBNext^® Ultra^™ II Directional RNA Library Prep Kit for Illumina^®
• NEBNext^® Ultra^™ II Directional RNA Library Prep with Sample Purification Beads
• NEBNext^® Ultra^™ II RNA Library Prep Kit for Illumina^®
• ProtoScript® II Reverse Transcriptase
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Nuclease-free Water
• Monarch® RNA Purification Columns
• Monarch^® Total RNA Miniprep Kit
• Monarch^® DNA/RNA Protection Reagent
• Monarch^® RNA Lysis Buffer
• Monarch^® RNA Wash Buffer
• Monarch^® gDNA Removal Columns
• Monarch^® Collection Tubes II
• Monarch^® Total RNA Miniprep Enzyme Pack

1. Electroporation of Cas12a RNP (ribonucleoprotein) into adherent cells using the Lonza 4D-Nucleofector®
2. Electroporation of Cas12a RNP (ribonucleoprotein) into adherent cells using the Neon® Electroporation
3. In vitro digestion of DNA with EnGen® Lba Cas12a (Cpf1) (NEB #M0653)
4. Quick Protocol for Total RNA Extraction (NEB #T2010)

• General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
• Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
• Guidelines for Purification of RNA from Cultured Mammalian Cells
• Guidelines for RNA Purification from Bacteria
• Guidelines for RNA Purification from Leukocytes (WBCs, PBMCs)
• Guidelines for RNA Purification from Plants
• Guidelines for RNA Purification from Tissues 
• Guidelines for RNA Purification from Whole Blood
• Guidelines for RNA Purification from Yeast

• Monarch Nucleic Acid Purification Brochure

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• T2013L_v1_10044094

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Monarch^® RNA Priming Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.