Monarch® DNA/RNA Protection Reagent is a component of the Monarch Total RNA Miniprep Kit. It is a solution for the preservation of nucleic acids in various tissue types. It is supplied as a 2X concentrate. This reagent does not need to be removed before RNA extraction with the Monarch Total RNA Miniprep Kit.
Figure 1: Monarch DNA/RNA Protection Reagent preserves RNA integrity for up to 1 month at 4°C
Samples were incubated in Monarch DNA/RNA Protection Reagent (NEB #T2011) at 4°C for the indicated time and subsequently processed using the Monarch Total RNA Miniprep Kit (NEB #T2010). Samples were eluted in 100 µl nuclease-free water. Rat liver and blood samples were diluted 5-fold (HeLa cells were not diluted), and 1 µl of each was loaded onto a Bioanalyzer Nano chip (HeLa and rat liver) or Pico chip (blood). The RIN values demonstrate the ability of the Protection Reagent to preserve RNA integrity over time.
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