Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with one kit!
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.
|Binding Capacity:||100 µg RNA|
|RNA Size:||≥ 20 nt|
|Purity:||A260/280 usually ≥ 1.8, A260/230 usually ≥ 1.8|
|Tissue Input:||up to 20 mg for high yield tissues|
|up to 50 mg for low yield tissues|
|Cultured Mammalian Cells and Blood Cells (PBMC's, WBC's) Input:||up to 1 x 107|
|Mammalian Whole Blood Input:||up to 3 ml|
|Bacteria Input:||up to 1 x 109|
|Yeast Input:||up to 5 x 107|
|Plant Input:||up to 100 mg|
|Elution Volume:||30 - 100 μl|
|Yield:||varies depending on sample type|
|Compatible downstream applications:||RNA Library prep for NGS, RT-PCR, RT-qPCR, Northern blots|
Validated Sample Types:
Additional Data Figures:
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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