Monarch® PCR & DNA Cleanup Kit (5 μg)
Quickly and easily purify high quality DNA from PCR and other enzymatic reactions.
- Elute in as little as 6 μl
- Prevent buffer retention and salt carry-over with optimized column design
- Save time with fast, user-friendly protocols
- No need to monitor pH
- Protocol modification allows for ssDNA purification, oligonucleotide purification, and purification of other small DNA fragments
- Buffers and columns available separately
- Significantly less plastic used when compared with other kits
- Responsibly-sourced and recyclable packaging
Monarch® PCR & DNA Cleanup Kit Protocol
The Monarch PCR & DNA Cleanup Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from PCR and other enzymatic reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging. The protocol can also be modified to enable the purification of smaller DNA fragments, including oligonucleotides and ssDNA.
View our videos on protocols, tips, and recycling Monarch.
APPLICATIONS PCR cleanup DNA from PCR reactions can be purified after amplification to remove polymerases, primers, detergents, dNTPs, etc. Enzymatic reaction cleanup Restriction enzymes and modifying enzymes such as ligases, kinases, nucleases, phosphatases are efficiently removed, allowing for effective desalting and concentration of the DNA sample. cDNA cleanup DNA/RNA complexes can be purified post-reverse transcription/amplification to enable removal of the RT and polymerase as well as nucleotides. Labeling cleanup Unincorporated radiolabeled or fluorescently labeled nucleotides can be removed from the DNA substrate Plasmid cleanup Plasmid preps from unknown sources may contain inhibitors and unwanted contaminants. Purification and concentration can be easily achieved using this kit. Oligonucleotide cleanup ssDNA oligonucleotides (≥ 18 nt) and dsDNA fragments (≥ 15 bp) can be purified using the Oligonucleotide Cleanup Protocol.
DNA Sample Type: DNA from PCR and other enzymatic reactions (e.g., restriction digests, kinase reactions, ligations).
ssDNA or dsDNA oligonucleotides from enzymatic reactions can also be purified using the Oligonucleotide Cleanup Protocol.
Binding Capacity: up to 5 μg DNA Size Range: ~50 bp to 25 kb
DNA ≥ 15 bp to 25 kb (dsDNA) and DNA ≥ 18 nt to 10 kb (ssDNA) can also be purified using the Oligonucleotide Cleanup Protocol.
DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
ssDNA ≥ 18 nt and dsDNA ≥ 15 bp: 70-85%
Elution Volume: ≥ 6 μl Purity: A260/280 > 1.8 and A260/230 > 1.8
Protocol Time: 5 minutes of spin and incubation time Compatible Downstream
ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
The following reagents are supplied with this product:
Store at (°C) Concentration Monarch® DNA Elution Buffer 25 1X Monarch® DNA Cleanup Binding Buffer 25 1X Monarch® DNA Wash Buffer 25 5X Monarch® DNA Cleanup Columns (5 μg) 25
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Monarch® PCR & DNA Cleanup Kit Protocol
Tips for using the Monarch® PCR & DNA Cleanup Kit
How to recycle your Monarch Kit components
NEB TV Ep. 6 – Sustainability
Quick Tips - Can I shorten the protocol for the Monarch® Cleanup?
Quick Tips - How do I apply my elution buffer to the Monarch® nucleic acid purification column?
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