Quickly and easily purify DNA from agarose gels with high yields.
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging
View our videos on protocols, tips, and recycling Monarch.
|DNA Sample Type:||double-stranded DNA from agarose gels|
|Binding Capacity:||up to 5 μg|
|DNA Size Range:||~50 bp to 25 kb|
|Typical Recovery:||DNA (50 bp to 10 kb): 70–90%
DNA (11–23 kb): 50–70%
|Elution Volume:||≥ 6 μl|
|Purity:||A260/280 > 1.8|
|Protocol Time:||10 minutes of spin and incubation time|
|ligation, restriction digestion, labeling and other enzymatic
manipulations, library construction and DNA sequencing.
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
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