CLIP-Biotin

CLIP-Biotin is suitable for applications such as biotinylation of CLIP-tag™ fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot or for capture with streptavidin for binding and interaction studies.

  • It is a cell-permeable substrate (BC-Biotin)
  • It is based on biotin with an amidocaproyl linker
Catalog # Concentration Size
S9221S Not Applicable 50 nmol
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  • Product Information
    This package contains 50 nmol of CLIP-Biotin substrate, sufficient to make 10 ml of a 5 μM solution for the labeling of CLIP-tag fusion proteins in cells.

    CLIP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal simultaneous labeling.

    There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of fusion proteins with CLIP-Biotin is described below.
    9147a_thumb
    Figure 1: Structure of CLIP-Biotin (MW 569.7 g/mol)
    Product Categories:
    Biotin Labels Products,
    CLIP-tag™ Substrates Products,
    Protein Labeling Products
    Applications:
    CLIP Cell,
    In vivo Imaging,
    Protein Localization
    ,
    Protein Labeling Snap Clip
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