SNAP-Vista® Green

This product has been discontinued.  The following substrates can be used in its place:
SNAP-Cell 505-Star (NEB #S9103)
SNAP-Surface 488 (NEB #S9124)
SNAP-Surface Alexa Fluor 488 (NEB #S9129)

  • Catalog # S9147 was discontinued on January 02, 2020

SNAP-Vista® Green is a green fluorescent substrate that can be used to label SNAP-tag® fusion proteins (in cell lysates or purified proteins) for detection by SDS-PAGE.

  • This substrate (BG-Vista Green) is based on fluorescein
  • It is optimized for excitation with the 488 nm laser excitation line in a laser based gel scanner
  • It can also be excited using 360 nm light from a standard UV-transilluminator
  • It has an excitation maximum at 500 nm and emission maxima at 524 nm

Storage Temperature:  -20°C

  • Product Information
    This package includes 50 nmol of SNAP-Vista Green substrate, sufficient to label one hundred 20 µl samples containing SNAP-tag fusion protein for in-gel detection. 

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate for detection by SDS-PAGE is described in this document.

    Figure 1: Excitation (dotted line) and emission spectra of SNAP-Vista Green coupled to SNAP-tag in buffer at pH 7.5.
    Figure 1: Excitation (dotted line) and emission spectra of SNAP-Vista Green coupled to SNAP-tag in buffer at pH 7.5.
    Figure 2: Typical SDS-PAGE gel from a gel scanner (Molecular Dynamics Storm Imager, 488 nm excitation)
    Lane 1 fluorescent MW marker 
    Lane 2-4 SNAP-His (22 kD) 0.1, 0.5, 1.0 µg 
    Lane 5-7 SNAP-CyA (40 kD) 0.1, 0.5, 1.0 µg 
    Lane 8-10 MBP-SNAP (63 kD) 0.1, 0.5, 1.0 µg
    Figure 3: Structure of SNAP-Vista Green (MW 628.6 g/mol)
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