SNAP-Capture Magnetic Beads
The SNAP-Capture Magnetic Beads are used to selectively immobilize and magnetically separate a SNAP-tag fusion protein from solution using magnetic agarose beads. They are prepared by the coupling of SNAP-tag® substrate benzylguanine with highly stable 20-100 µm superparamagnetic particles.
- These beads show a low non-specific absorption of proteins from a complex lysate
- They are suitable for pull down applications
Two ml of SNAP-Capture Magnetic Beads is sufficient to perform 25 immobilization assays using 80 µl of bead suspension per assay.
The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule or modified surface to a protein of interest. The SNAP-tag is a protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzyl purines and benzyl pyrimidines. In the immobilization reaction, the SNAP-tag is covalently attached to the substituted benzyl group of the SNAP-Capture Magnetic Beads.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and capture and immobilization of the fusion protein using SNAP-Capture Magnetic Beads.
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