SNAP-Surface® Alexa Fluor® 546

SNAP-Surface® Alexa Fluor® 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells.

  • This is a cell impermeable substrate based on Alexa Fluor 546
  • It is suitable for standard TAMRA or Cy3 filter sets
  • It has an excitation maximum at 558 nm and emission maximum at 574 nm

Ordering Information

  • Product Information
    This package includes 50 nmol of SNAP-Surface Alexa Fluor 546 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution. 

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. 

    There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1: Live COS-7 cells transiently transfected with pSNAP-ADRβ2

    Figure 1: Live COS-7 cells transiently transfected with pSNAP-ADRβ2
    Cells were labeled with SNAP-Surface Alexa Fluor® 546 (red).
    Figure 2: Excitation (dotted line) and emission (full line) spectra of SNAP-Surface Alexa Fluor® 546 coupled to SNAP-tag in buffer at pH 7.5
    Figure 2: Excitation (dotted line) and emission (full line) spectra of SNAP-Surface Alexa Fluor® 546 coupled to SNAP-tag in buffer at pH 7.5
    Product Categories:
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    Snap Surface Products,
    In vivo Imaging,
    Pulse Chase
    Receptor Internalization
    Protein Localization
    Protein Labeling Snap Clip
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