SNAP-Surface® 532

  • Catalog # S9131 was discontinued on January 01, 2013
  • Product Information
    SNAP-Surface® 532 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This substrate (BG-532) is based on the ATTO-TEC dye ATTO 532 and is suitable for standard rhodamine filter sets. It has an excitation maximum at 536 nm and emission maximum at 554 nm. This package includes 50 nmol of SNAP-Surface 532 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1:  
    Live CHO-K1 cells transiently transfected with pSNAP-ADRβ2. Cells were labeled with SNAP-Surface 532 (red) and counterstained with Hoechst 33342 (blue).
    Figure 2:
    Excitation (dotted line) and emission spectra of SNAP-Surface 532 coupled to SNAP-tag in buffer at pH 7.5
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