SNAP-Cell® Oregon Green®

SNAP-Cell® Oregon Green® is a photostable green fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells or in vitro. Conjugates of Oregon Green are more photostable than those of fluorescein, and their fluorescence properties are essentially pH insensitive in the physiological pH range.

  • It is a cell-permeable substrate (BG-Oregon Green) based on the Invitrogen dye, Oregon Green
  • It is suitable for standard fluorescein filter sets
  • It has an excitation maximum at 490 nm and an emission maximum at 514 nm

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  • Product Information
    This package contains 50 nmol of SNAP-Cell Oregon Green substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

    The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the dye-substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag. 
     
    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below. 

    Figure 1: Live U-2 OS cells transiently transfected with pSNAP-Cox8A (mitochondrial expression).

    Figure 1: Live U2OS cells transiently transfected with pSNAP-Cox8A (mitochondrial expression).
    Cells were labeled with SNAP-Cell Oregon Green (green) for 15 minutes and counterstained with Hoechst 33342 (blue).
    Figure 2: 
    Excitation (dotted line) and emission spectra after coupling of SNAP-Cell Oregon Green to SNAP-tag in buffer at pH 7.5.
    Figure 2:  Excitation (dotted line) and emission spectra after coupling of SNAP-Cell Oregon Green to SNAP-tag in buffer at pH 7.5.

    Product Categories:
    SNAP-tag Substrates Products ,
    Protein Labeling Products
    Applications:
    In vivo Imaging,
    Protein Localization,
    Protein Labeling Snap Clip
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