SNAP-Cell® 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro.
- It is a cell-permeable substrate (also termed SiR-SNAP) based on 6-carboxy-tetramethylsiliconrhodamine
- It is suitable for standard Cy5 filter sets
- It has an excitation maximum at 645 nm and an emission maximum at 661 nm
This package contains 30 nmol of SNAP-Cell 647-SiR substrate, sufficient to make 10 ml of a 3 μM SNAP-tag fusion protein labeling solution.
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.
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