This kit contains an affinity matrix used for the isolation of mRNA containing polyadenylic (poly A) regions (1) from purified total RNA. This matrix consists of oligo (dT)25 covalently coupled to a cross-linked cellulose bead. This product is guaranteed for twenty-four months when stored at 4°C in the supplied storage buffer.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|5 M NaCl||500 mM|
|3M NaAC||300 mM|
|Wash Buffer I||1 X|
|Wash Buffer (15 ml)||1 X|
|Elution Buffer||1 X|
|Low Salt Buffer||1 X|
|Glycogen Solution||10 mg/ml|
|microcentrifuge tubes containing oligo (dT)25 -cellulose beads|
|microcentrifuge spin columns|
|sterile microcentrifuge tubes|
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