Protein A Magnetic Beads

Protein A Magnetic Beads are an affinity matrix for the small-scale isolation and purification of immunoglobulins. Specifically, the beads consist of a truncated form of recombinant Protein A that is covalently coupled to a nonporous superparamagnetic particle. 

  • Exhibits high affinity for subclasses of IgG from many species including human, rabbit and mouse
  • The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants 
  • The matrix can be regenerated without loss of binding capacity
  • Can be used to immunoprecipitate target proteins from crude cell lysates using a selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, thereby avoiding the co-elution of antibody with the target antigen 
  • Binding capacity: 1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG
  • Quick, small-scale purification of many subclasses of IgGs affords higher throughput and efficiency
 
Learn more about how this product is being used in the Product Citation Tool
2019-20_Catalog_Promo_0519
Catalog # Concentration Size
S1425S Not Applicable 1 ml
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