Random Primer 6

  • Random Primer 6 is a mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA
  • Ensures the presence of virtually all sequence combination of hexamer primers
  • Used to screen gene libraries
  • Used to probe northern and southern blots
  • Used for in situ hybridizations
  • Sequence: 5' d(N6 ) 3' [N=A,C,G,T]
Learn more about how this product is being used in the Product Citation Tool
Catalog # Concentration Size
S1230S Not Applicable 1 A260 units
To find out how to order this product from your current location, click the button below:
International Customer & Order Support
Please enter a quantity for at least one size
  • Product Information
    This mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate nonradioactive nucleotide in the reaction mixture. Use of synthetic d(N)6 primer ensures the presence of virtually all sequence combination of hexamer primers which results in equally labelled DNA of high specific activity (1,2). Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

    Sequence

    5' d(N6 ) 3' [N=A,C,G,T]
    Product Categories:
    Reverse Transcriptases & RT-PCR Products
    Applications:
    RT-PCR & cDNA Synthesis,
    RT-qPCR, RT-PCR and cDNA Synthesis
    • Properties & Usage
    • Product Notes
    • References
  • Protocols, Manuals & Usage
  • Citations & Technical Literature
    • Citations
  • Quality, Safety & Legal
    • Quality Control Assays
    • Specifications
    • Certificate of Analysis
    • Safety Data Sheets
    • Datacards
    • Legal and Disclaimers
Loading Spinner